EMAGE mouse gene expression spatial database

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EMAGE:5182

Tnni3 troponin I, cardiac 3 ( MGI:98783)
TS15 (9.5 dpc)
in situ hybridisation

Data Images


Fig7A. Di Lisi R; Millino C; Calabria E; Altruda F; Schiaffino S; Ausoni S, "Combinatorial cis-acting elements control tissue-specific activation of the cardiac troponin I gene in vitro and in vivo." J Biol Chem 1998;273(39):25371-80 [PMID:9738004]

Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

5182_wholemount_moderate.wlz

5182_wholemount_notDetected.wlz

5182_wholemount_strong.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:5182_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

heart

detected

Annotation Validation:

EMAGE Editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	Tnni3 probeA
Entity Detected:	Tnni3, troponin I, cardiac 3 ( MGI:98783)
Sequence:	sense strand is shown >Tnni3 probeA CGCTTGAGCAGCACAGAGGGGACCCAACCCTCCAAGGTCACCAGGGACACCCTTCTAAGACCCTCCAGGA ATCTGCAATCCCATTCTCTACCTCTGGAGATCAGCATGGCGGATGAGAGCAGCGATGCGGCTGGGGAACC CCAGCCGGCGCCTGCTCCTGTCCGACGTCGCTCCTCGGCCAACTACCGAGCCTATGCCACCGAGCCACAT GCCAAGAAAAAGTCTAAGATCTCCGCCTCCAGAAAACTTCAGTTGAAGACTCTGATGCTGCAGATTGCGA AGCAGGAGATGGAGCGTGAGGCAGAGGAGCGACGTGGAGAGAAGGGCCGCGTTCTGAGCACGCGTTGCCA GCCCTTGGTGTTGGATGGGCTGGGCTTTGAAGAGCTTCAGGACCTATGCCGGCAGCTTCACGCTCGTGTG GACAAAGTGGATGAAGAGAGATACGACGTGGAAGCAAAAGTCACCAAGAACATCACTGAGATTGCAGATC TGACCCAGAAGATCTATGACCTGCGTGGCAAGTTTAAGCGGCCCACTCTCCGAAGAGTGAGAATCTCAGC AGATGCCATGATGCAGGCACTACTGGGGACCCGGGCCAAGGAATCCTTGGACCTGAGGGCCCACCTCAAG CAGGTGAAGAAGGAGGACATTGAGAAGGAAAACCGGGAGGTGGGAGACTGGCGCAAGAATATCGATGCAC TAAGTGGAATGGAAGGCCGAAAGAAAAAGTTTGAGGGCTGAGCCCATGGCTCTCACACTGTGCTCTGAAG GGCATCTCTGAGGAATAAATTTCTTTAAACTGGAAAAAAAAAAAAAAAAAAAAAAAAAAA
	nt 1 - nt 830 of NM_017144.1
Notes:	The Tnni3 (cTNI) probe template used in this study by Di Lisi et al., 1998 [PMID:9738004] is described as follows: "An 800-base pair DNA fragment corresponding to the rat cTNI clone (Ausoni et al, 1991 [PMID:1935696] was used to produce sense and antisense RNAs". Editors note: Ausoni et al, 1991 published the sequence of a 830nt rat cardiac troponin I clone (RCTI-5 - see Figs 1 and 2 therein and also NM_017144.1) which is presumed to correspond to the probe sequence. Note however that Ausoni et al synthesised their antisense in situ probe from this clone when linearised internally using MluI (cuts at nt 340) which resulted in a ~500nt probe.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Age:	9.5 dpc
Theiler Stage:	TS15
Mutations:	none (wild-type)
Preparation:	wholemount

Procedures

Staining procedure:

alkaline phosphatase + BMpurple

General Information

Authors:	Di Lisi R; Millino C; Calabria E; Altruda F; Schiaffino S; Ausoni S. 1998 [PMID:9738004] . Indexed and spatially mapped by EMAGE
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	non-screen
References:	[ doi:10.1074/jbc.273.39.25371] [ PMID:9738004] Di Lisi R, Millino C, Calabria E, Altruda F, Schiaffino S, Ausoni S 1998 Combinatorial cis-acting elements control tissue-specific activation of the cardiac troponin I gene in vitro and in vivo. J Biol Chem (273):25371-80
	[ PMID:1935696] Ausoni S, De Nardi C, Moretti P, Gorza L, Schiaffino S 1991 Developmental expression of rat cardiac troponin I mRNA. Development (112):1041-51
Links:	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source