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EMAGE:5194

Ube2i ubiquitin-conjugating enzyme E2I ( MGI:107365)
TS17 (11.0 dpc)
in situ hybridisation

Data Images
EMAGE:5194
Fig 2D-11dpc Copyright: Reprinted with permission from Elsevier from [doi:10.1016/S0925-4773(00)00402-0] Mech Dev 97: 3-12, Machon O; Backman M; Julin K; Krauss S, " Yeast two-hybrid system identifies the ubiquitin-conjugating enzyme mUbc9 as a potential partner of mouse Dac" Copyright 2000. [PMID:11025202]

Expression pattern clarity: two stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:5194Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
5194_wholemount__moderate.wlz
5194_wholemount__possible.wlz
5194_wholemount__notDetected.wlz
5194_wholemount__strong.wlz
(what is wlz format?)
Download all expression domains: EMAGE:5194_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
forelimb bud mesenchyme
detected detected
regionalmUbc9 expression is detected in the proliferation zone of the fore limb at 11 dpc
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:Ube2i probeA
Entity Detected:Ube2i, ubiquitin-conjugating enzyme E2I ( MGI:107365)
Notes:The Ube2i (Ubc9) probe template used in this study by Machon et al., 2000 [PMID:11025202] was "full-length mUbc9 in pCI-neo", which "was linearized with EcoRI and transcribed with T3 RNA polymerase". Editors Note: the full sequence or identity of the "full-length" clone is not reported.
Chemistry:RNA
Strand:antisense
Specimen
Organism:mouse
Age:11.0 dpc
Theiler Stage:TS17
Mutations:none (wild-type)
Preparation:wholemount
Procedures
General Information
Authors:Machon O; Backman M; Julin K; Krauss S. 2000 [PMID:11025202] . Indexed and spatially mapped by EMAGE
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1016/S0925-4773(00)00402-0] [ PMID:11025202] Machon O, Backman M, Julin K, Krauss S 2000 Yeast two-hybrid system identifies the ubiquitin-conjugating enzyme mUbc9 as a potential partner of mouse Dac. Mech Dev (97):3-12
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE