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EMAGE:5197

Unc5b unc-5 homolog B (C. elegans) ( MGI:894703)
TS17 (10.5 dpc)
in situ reporter

Data Images
EMAGE:5197
Fig1E [doi:10.1038/nature03080] Lu X; Le Noble F; Yuan L; Jiang Q; De Lafarge B; Sugiyama D; Breant C; Claes F; De Smet F; Thomas JL; Autiero M; Carmeliet P; Tessier-Lavigne M; Eichmann A "The netrin receptor UNC5B mediates guidance events controlling morphogenesis of the vascular system." 2004 Nature 432:179-86. [PMID:15510105] © Nature Publishing Group. Reproduced with permission from Nature.

Expression pattern clarity: two stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
X-gal staining at E10.5 of Unc5b+/- mice. Note expression in dorsal aorta (ao), internal carotid artery (ica) and intersomitic arteries (arrowheads). Neural expression of Unc5b is restricted to the otic placode (o) and eye (e). h, heart.
Expression Pattern Description
Spatial Annotation:
EMAGE:5197Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
5197_wholemount__moderate.wlz
5197_wholemount__weak.wlz
5197_wholemount__notDetected.wlz
5197_wholemount__strong.wlz
(what is wlz format?)
Download all expression domains: EMAGE:5197_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
dorsal aorta
detected detected
internal carotid artery
detected detected
arterial system
detected detected
regionalExpression is detected in intersomitic arteries
otocyst
detected detected
Neural expression of Unc5b is restricted to the otic placode and eye.
eye
detected detected
Neural expression of Unc5b is restricted to the otic placode and eye.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ reporter
Entity Detected:Unc5b, unc-5 homolog B (C. elegans) ( MGI:894703)
Notes:Unc5b expression was assayed in this study by Lu et al., 2004 [PMID:15510105] in embryos heterozygous for a targeted allele where a secretory trap vector possessing a splice acceptor followed by a linker protein, a transmembrane domain, beta-geo, an IRES domain, and a placental alkaline phosphatase reporter gene was targeted to intron 2 of the gene locus. As a result of integration, exons 1 and 2 splice to the vector sequences resulting in a mutant fusion protein with no biological activity.
Labelled with:LacZ
Specimen
Organism:mouse
Strain:involves: 129P2/OlaHsd
Age:10.5 dpc
Theiler Stage:TS17
Mutations:Unc5btm1Matl/Unc5b+
Preparation:wholemount
Procedures
General Information
Authors:Lu X; Le Noble F; Yuan L; Jiang Q; De Lafarge B; Sugiyama D; Breant C; Claes F; De Smet F; Thomas JL; Autiero M; Carmeliet P; Tessier-Lavigne M; Eichmann A. 2004 [PMID:15510105] . Indexed and spatially mapped by EMAGE
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1038/nature03080] [ PMID:15510105] Lu X, Le Noble F, Yuan L, Jiang Q, De Lafarge B, Sugiyama D, Breant C, Claes F, De Smet F, Thomas JL, Autiero M, Carmeliet P, Tessier-Lavigne M, Eichmann A 2004 The netrin receptor UNC5B mediates guidance events controlling morphogenesis of the vascular system. Nature (432):179-86
Links:MGI:3760978 allele description
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE