EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:5202

Vcan versican ( MGI:102889)
TS15 (9.5 dpc)
in situ hybridisation

Data Images


Fig 2A-wt Copyright: Reprinted with permission from Elsevier from [doi:10.1016/S0925-4773(97)00151-2] Mech Dev 69(2): 39-51, Henderson DJ; Ybot-Gonzalez P; Copp AJ, Over-expression of the chondroitin sulphate proteoglycan versican is associated with defective neural crest migration in the Pax3 mutant mouse (splotch) Copyright 1997. [PMID:9486530]	Fig 2B Copyright: Reprinted with permission from Elsevier from [doi:10.1016/S0925-4773(97)00151-2] Mech Dev 69(2): 39-51, Henderson DJ; Ybot-Gonzalez P; Copp AJ, Over-expression of the chondroitin sulphate proteoglycan versican is associated with defective neural crest migration in the Pax3 mutant mouse (splotch) Copyright 1997. [PMID:9486530]	Fig 2D Copyright: Reprinted with permission from Elsevier from [doi:10.1016/S0925-4773(97)00151-2] Mech Dev 69(2): 39-51, Henderson DJ; Ybot-Gonzalez P; Copp AJ, Over-expression of the chondroitin sulphate proteoglycan versican is associated with defective neural crest migration in the Pax3 mutant mouse (splotch) Copyright 1997. [PMID:9486530]

Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Notes:

In (A) versican expression in the mesoderm of the occipital/cervical region (arrowheads). Palnes of sections (B) and (D) are indicated. Abbreviations: a, atrial chamber; f, foregut; h, hindgut; n, neural tube; p, presomitic mesoderm; v, ventricular chamber.

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

5202_wholemount__moderate.wlz

5202_wholemount__weak.wlz

5202_wholemount__possible.wlz

5202_wholemount__notDetected.wlz

5202_wholemount__strong.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:5202_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

mesenchyme

detected

regional

Expression is detected in the mesoderm of the occipital/cervical region

trunk somite

detected

caudal neuropore

detected

hindgut

detected

Annotation Validation:

EMAGE Editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	Vcan probeA
Entity Detected:	Vcan, versican ( MGI:102889)
Notes:	The Vcan probe used in this study by Henderson et al., 1997 [PMID:9486530] was "the V1 probe", or "probeA" which was amplified "from E10.5 CBA/Ca embryos. Primers were designed to amplify portions of the V1 splice form of mouse versican (see Fig. 1). Amplification between base pairs 6919-6939 and 7337-7357 of the V1 transcript generated probe A".
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Age:	9.5 dpc
Theiler Stage:	TS15
Mutations:	none (wild-type)
Preparation:	wholemount

Procedures

Staining procedure:

alkaline phosphatase + NBT/BCIP

General Information

Authors:	Henderson DJ; Ybot-Gonzalez P; Copp AJ . 1997 [PMID:9486530] . Indexed and spatially mapped by EMAGE
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	non-screen
References:	[ doi:10.1016/S0925-4773(97)00151-2] [ PMID:9486530] Henderson DJ, Ybot-Gonzalez P, Copp AJ 1997 Over-expression of the chondroitin sulphate proteoglycan versican is associated with defective neural crest migration in the Pax3 mutant mouse (splotch). Mech Dev (69):39-51
Links:	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source