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EMAGE:5203

Vcan versican ( MGI:102889)
TS17 (10.5 dpc)
in situ hybridisation

Data Images
EMAGE:5203 EMAGE:5203 EMAGE:5203 EMAGE:5203
Fig 3A-wt Copyright: Reprinted with permission from Elsevier from [doi:10.1016/S0925-4773(97)00151-2] Mech Dev 69(2): 39-51, Henderson DJ; Ybot-Gonzalez P; Copp AJ, Over-expression of the chondroitin sulphate proteoglycan versican is associated with defective neural crest migration in the Pax3 mutant mouse (splotch) Copyright 1997. [PMID:9486530] Fig 3B Copyright: Reprinted with permission from Elsevier from [doi:10.1016/S0925-4773(97)00151-2] Mech Dev 69(2): 39-51, Henderson DJ; Ybot-Gonzalez P; Copp AJ, Over-expression of the chondroitin sulphate proteoglycan versican is associated with defective neural crest migration in the Pax3 mutant mouse (splotch) Copyright 1997. [PMID:9486530] Fig 3D Copyright: Reprinted with permission from Elsevier from [doi:10.1016/S0925-4773(97)00151-2] Mech Dev 69(2): 39-51, Henderson DJ; Ybot-Gonzalez P; Copp AJ, Over-expression of the chondroitin sulphate proteoglycan versican is associated with defective neural crest migration in the Pax3 mutant mouse (splotch) Copyright 1997. [PMID:9486530] Fig 3F Copyright: Reprinted with permission from Elsevier from [doi:10.1016/S0925-4773(97)00151-2] Mech Dev 69(2): 39-51, Henderson DJ; Ybot-Gonzalez P; Copp AJ, Over-expression of the chondroitin sulphate proteoglycan versican is associated with defective neural crest migration in the Pax3 mutant mouse (splotch) Copyright 1997. [PMID:9486530]

Expression pattern clarity: two stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
(A) Wild type embryos express versican in the occipital/cervical and cervical mesoderm as well as in the caudal part of each somite (arrowheads). (B,D) Transverse vibratome sections through the upper cervical (B) and fore limb bud (D) regions of the whole-mount wild type embryo. (F) Longitudinal vibratome section through a wild type embryo (region bracketed in (A)) demonstrating the expression of versican confined to the caudal part of each somite. Arrowheads indicate inter-somitic clefts. Anterior is to the left, posterior to the right. Abbreviations: d, dermomyotome; g, dorsal root ganglia; n, neural tube; s, sclerotome.
Expression Pattern Description
Spatial Annotation:
EMAGE:5203Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
5203_wholemount__moderate.wlz
5203_wholemount__weak.wlz
5203_wholemount__notDetected.wlz
5203_wholemount__strong.wlz
(what is wlz format?)
Download all expression domains: EMAGE:5203_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
mesenchyme
detected detected
regionalVersican expression is detected in the occipital/cervical and cervical mesoderm
trunk somite
detected detected
regionalExpressed in the caudal part of each somite. The dermomyotomes exclude versican expression.
dorsal root ganglion
not detected not detected
homogeneousThe dorsal root ganglia exclude versican expression
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:Vcan probeA
Entity Detected:Vcan, versican ( MGI:102889)
Notes:The Vcan probe used in this study by Henderson et al., 1997 [PMID:9486530] was "the V1 probe", or "probeA" which was amplified "from E10.5 CBA/Ca embryos. Primers were designed to amplify portions of the V1 splice form of mouse versican (see Fig. 1). Amplification between base pairs 6919-6939 and 7337-7357 of the V1 transcript generated probe A".
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Age:10.5 dpc
Theiler Stage:TS17
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Henderson DJ; Ybot-Gonzalez P; Copp AJ . 1997 [PMID:9486530] . Indexed and spatially mapped by EMAGE
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1016/S0925-4773(97)00151-2] [ PMID:9486530] Henderson DJ, Ybot-Gonzalez P, Copp AJ 1997 Over-expression of the chondroitin sulphate proteoglycan versican is associated with defective neural crest migration in the Pax3 mutant mouse (splotch). Mech Dev (69):39-51
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE