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EMAGE:5204

Gramd3 GRAM domain containing 3 ( MGI:1914815)
TS20 (12.5 dpc)
in situ hybridisation

Data Images
EMAGE:5204 EMAGE:5204 EMAGE:5204 EMAGE:5204 EMAGE:5204
Raw signal output, as movie. All sections along the X-axis, as movie. All sections along the Y-axis, as movie. All sections along the Z-axis, as movie. Photograph prior to 3D imaging.
EMAGE:5204 EMAGE:5204
Photograph prior to 3D imaging. Sample morphology following 3D imaging, as movie

View 3D opt image: EMAGE genex expression entry
Download 3D images in woolz format
Expression pattern clarity: no stars
Notes:
This is a traditionally produced wholemount in situ hybridisation stained using alkaline phosphatase + BM Purple. It has been imaged using Optical Projection Tomography. The visible channel depicting the signal has been false-coloured green and the fluorescence channel depicting autofluorescence has been false-coloured blue. The staining seen here in the wholemount images and the signals in the OPT brightfield scan are the result of overdevelopment of the colour reaction and do not represent true gene expression.
Expression Pattern Description
Text Annotation:
StructureLevelPatternNotes
embryo
not detected not detected
homogeneous
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:Gramd3_probeA
Entity Detected:Gramd3, GRAM domain containing 3 ( MGI:1914815)
Sequence:sense strand is shown

>Gramd3_probeA
ATCCTGGTCAGCCATCAGACCTAAGGGGTACACTGTTTACCAGCAAACTCAAAATGGTCCTTTAGAGAAT
TAACCACAAACAAGGCAAGAGCCTTCAACAAGGTGGTGAATATCTCCTCCTGAATAAGCTTGCTTCCAAC
CCCTGTATTTTTGTGGGTTTTTATTTTCCCCTTTCTACTCTTATGTTCTGATTTCTTTCATCTATGCCTT
TTAATTTCCCAATTCAGTTTTGTGAGCCTGCCCGGTAAAGTGAATTAAGCATCCCATCTGCGTTGTGCTG
TCTTGTTCCCGTGACGAGTACTTAATTGAAGTGATTCTCCTCAGCCTCCCGTCAGGAATTTTTGATCACA
TTCAGTTTAAATCACATTCACTCTGAAACCAAAACTAATCTTTCAGCCAAAAGTTGTCATAATGATTTAA
CATGGGACGGAAAGC
nt 56662645 - nt 56663079 of NC_000084.5
Notes:This probe was transcribed from template produced by a PCR of whole genomic DNA using primers incorporating T3 and T7 promotors. The antisense probe was transcribed using the T7 polymerase. Primers: 5' AATTAACCCTCACTAAAGGATCCTGGTCAGCCATCAGAC 3' 5' TAATACGACTCACTATAGGGCTTTCCGTCCCATGTTAAA 3' Product length: 435bp
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:CD-1
Age:12.5 dpc
Theiler Stage:TS20
Mutations:none (wild-type)
Preparation:opt
Procedures
Fixation:2% paraformaldehyde
Embedding:agarose
Staining procedure:alkaline phosphatase + BMpurple
General Information
Authors:Peter Branney, David R FitzPatrick
Principal investigator:David R FitzPatrick, Medical Research Council, Human Genetics Unit MRC Western General Hospital Crewe Road, Edinburgh, Scotland EH4 2XU
Submitted by:Peter Branney, Medical Research Council, Human Genetics Unit MRC Western General Hospital Crewe Road, Edinburgh, Scotland EH4 2XU
Experiment type:screen
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceFACEBASE