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EMAGE:5205

Vcp valosin containing protein ( MGI:99919)
TS14 (9.5 dpc)
immunohistochemistry

Data Images
EMAGE:5205
Fig7a of Muller JM; Meyer HH; Ruhrberg C; Stamp GW; Warren G; Shima DT. 1999 "The mouse p97 (CDC48) gene. Genomic structure, definition of transcriptional regulatory sequences, gene expression, and characterization of a pseudogene" J Biol Chem 274:10154-62. [PMID:10187799]

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:5205Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
5205_wholemount__moderate.wlz
5205_wholemount__weak.wlz
5205_wholemount__possible.wlz
5205_wholemount__notDetected.wlz
5205_wholemount__strong.wlz
(what is wlz format?)
Download all expression domains: EMAGE:5205_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
embryo
detected detected
Staining was observed throughout the embryo, but was highest in limb buds, tail bud, branchial arches and somites.
limb
strong strong
Staining was observed throughout the embryo, but was highest in limb buds, tail bud, branchial arches and somites.
tail bud
strong strong
Staining was observed throughout the embryo, but was highest in limb buds, tail bud, branchial arches and somites.
branchial arch
strong strong
Staining was observed throughout the embryo, but was highest in limb buds, tail bud, branchial arches and somites.
head somite
strong strong
Staining was observed throughout the embryo, but was highest in limb buds, tail bud, branchial arches and somites.
trunk somite
strong strong
Staining was observed throughout the embryo, but was highest in limb buds, tail bud, branchial arches and somites.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:antibody
Identifier:Vcp antibody A
Entity Detected:Vcp, valosin containing protein ( MGI:99919)
Notes:The anti-VCP (p97) antisera used in this study by Muller et al., 1999 [PMID:10187799] was one of two possible antisera. The authors state "Anti-p97 antibodies N2 and N5 were raised in rabbit against two bacterially expressed fusion proteins consisting of glutathione S-transferase (GST) fused to fragments of mouse p97 (N2: amino acids 2-186; N5: amino acids 200-461). The antibodies were affinity-purified using full-length recombinant His-tagged p97 and were shown to uniquely recognize p97 in Western blot analyses of rat liver homogenate (data not shown). Immunohistochemical results obtained using N2 or N5 were indistinguishable". Editors note: it is not specified if N2 or N5 was used to generate the data shown here.
Antibody Type:polyclonal
Raised In:rabbit
Specimen
Organism:mouse
Age:9.5 dpc
Theiler Stage:TS14
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:3% formaldehyde
Secondary Antibody:biotinylated secondary antibody
Tertiary Antibody:strepavidin
Labelled with:horse radish peroxidase
Visualisation method:DAB
General Information
Authors:Muller JM; Meyer HH; Ruhrberg C; Stamp GW; Warren G; Shima DT. 1999 [PMID:10187799] . Indexed and spatially mapped by EMAGE
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1074/jbc.274.15.10154] [ PMID:10187799] Muller JM, Meyer HH, Ruhrberg C, Stamp GW, Warren G, Shima DT 1999 The mouse p97 (CDC48) gene. Genomic structure, definition of transcriptional regulatory sequences, gene expression, and characterization of a pseudogene. J Biol Chem (274):10154-62
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE