EMAGE mouse gene expression spatial database

EMAGE:5212

Wasf1 WASP family 1 ( MGI:1890563)
TS15 (9.0 dpc)
in situ reporter

Data Images


Fig5-E9 Dahl JP; Wang-Dunlop J; Gonzales C; Goad ME; Mark RJ; Kwak SP "Characterization of the WAVE1 knock-out mouse: implications for CNS development." 2003 J Neurosci 23(8):3343-52 [PMID:12716942]

Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

wholemount mapping

Download individual expression domains:

Download all expression domains:

Find spatially similar wholemount expression patterns:

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

embryo

detected

homogeneous

In E9 embryos, WAVE1 is expressed throughout the entire embryo as measured by beta-galactosidase activity

Annotation Validation:

EMAGE Editor

Detection Reagent

Type:	in situ reporter
Entity Detected:	Wasf1, WASP family 1 ( MGI:1890563)
Notes:	Wasf1 (Wave1) expression in this study by Dahl et al., 2003 [PMID:12716942] was assayed in embryos heterozygous for a gene trapped allele where the gene trap vector (SA-Bgeo-pA-PGK-BTK-SD) inserted in intronic sequence approximately 20 bp upstream of the endogenous start codon in exon 2. Two transcripts were identified by 5' RACE PCR, each arising from one of the two untranslated exons (1a and 1b). Protein was undetected by Western blot analysis of homozygous mutant cerbral cortex and hippocampus tissues. The pattern of beta-galactosidase activity was similar to the expression pattern of the endogenous gene.
Labelled with:	LacZ

Specimen

Procedures

Fixation:

4% paraformaldehyde

General Information

Authors:	Dahl JP; Wang-Dunlop J; Gonzales C; Goad ME; Mark RJ; Kwak SP. 2003 [PMID:12716942] Indexed and spatially mapped by EMAGE
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	non-screen
References:	[ PMID:12716942] Dahl JP, Wang-Dunlop J, Gonzales C, Goad ME, Mark RJ, Kwak SP 2003 Characterization of the WAVE1 knock-out mouse: implications for CNS development. J Neurosci (23):3343-52
Links:	MGI:2663235 allele description
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source