Type: | in situ hybridisation probe |
Identifier: | MGI:3694220 |
Entity Detected: | Wdr12, WD repeat domain 12 ( MGI:1927241) |
Sequence: | sense strand is shown
>MGI:3694220
GATTATCAACGCACACATTTTCGTGGAGAAGTCTCGTGTGTATATTCAGCCATGGCACAACTCCAGGCAC
GTTTCTACAGCGAGAACAAGAAATACGCAGTGGATGATGTCCCTTTCTCAATCCCTGCCGCTGCTGAAGT
TGCGGACCTTAGTAACATTATCAATAAGTTGCTGGAGACCAAAAATGAACTCCACAAGCATGTCGAGTTT
GATTTCCTTATCAAGGGCCAGTTTCTTCGAGTGCCCTTGGTCAAACACATGGAACTGGAGAACATTTCAT
CGGAAGAGGTTGTGGAACTAGAATATGTGGAAAAGTACACCGCACCCCAGCCAGAGCAGTGCATGTTCCA
TGATGACTGGATCAGCTCAATTGAAGGGGCAGAAGAATGGATCCTGTCTGGTTCTTATGATAAGACTTCT
CGGATCTGGTCCTTGGAAGGAAAGTCGATAATGACAATTGTGGGACATACAGATGTTGTGAAAGATGTGG
CTTGGGTGAAAAAAGACAGTTTGTCCTGCTTACTACTGACGGCCTCGATGGACCAGACTGTTCTCTTATG
GGAGTGGAATGTAGAGAAGAACAAAGTGAAAGCTCTGCACTGCTGCCGAGGGCATGCTGGGAGTGTGGAC
GCCATCGCAGTCGATAGCTCGGGAGCTAAGTTTTGCAGTGGCTCCTGGGATAAGATGCTAAAGATCTGGT
CTACAGTCCCTACAGATGAAGAAGATGAAATGGAAGAAGCCACAAATCGACCAAGAAAAAAGCAAAAAAC
AGAACAATTGGGACTAACAAGAACACCCTTGGTGACCCTCTCTGGCCACACAGAAGCAATTTCCTCAGTC
CTTTGGTCAGACGCTGAAGAAATCTGTAGTGCATCTTGGGATCACACAATTAGAGTGTGGGATGTTGAGT
CTGGCGGTCTTAAGTCAACTCTGACAGGAAATAAAGTGTTTAATTGTATTTCCTATTCTCCCCTTTGTAA
ACGTTTGGCATCTGGAAGCACAGACAGGCATATTAGACTGTGGGATCCCCGAACTAAAGATGGTTCTTTG
GTGTCGCTGTCTCTCACCTCACACACAGGCTGGGTGACATCAGTGAAGTGGTCTCCTACCCATGAACAGC
AGCTCATCTCGGGCTCTTTAGATAACATTGTGAAGCTATGGGATACAAGAAGCTGTAAGGCTCCTCTCTA
CGATCTAGCTGCTCATGAAGACAAAGTTCTAAGTGTGGACTGGACAGACACAGGGCTACTTCTGAGTGGA
GGAGCAGACAATAAATTGTATTCCTACAGCTATTCCCCTACCGCTTCCCATGTGGGGGCTTGAAAATGAA
TGGCTGAGAAGAGAAA
|
| nt 1 - nt 1346 of AY059431.1 |
Notes: | The Wdr12 probe used in this study by Nal et al., 2002 [PMID:11827460] was "prepared by in vitro transcription using the linearized Wdr12 cDNA (cloned into the pGEMt-easy vector; Promega, Madison, WI) in the presence of digoxygenin (DIG)-coupled UTP as described by the manufacturer (Boehringer Mannheim, Meylan, France). Production of complementary antisense and sense probes used ApaI-linearized cDNA and SP6 RNA polymerase, and NdeI-linearized cDNA and T7 RNA polymerase, respectively".
Editors Note: see Fig 1 therein and AY059431.1 for the sequence of the Wdr12 cDNA referred to by the authors. There are no ApaI or NdeI sites in this sequence indicating linearisation occurred within the vector. |
Chemistry: | RNA |
Strand: | antisense |
Label: | digoxigenin |