Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:5222

Edn3 endothelin 3 ( MGI:95285)
TS18 (10 dpc)
in situ hybridisation

Data Images
EMAGE:5222
Fig3left. from Gut 1999 44(2): 246-52, Leibl MA; Ota T; Woodward MN; Kenny SE; Lloyd DA; Vaillant CR; Edgar DH, Expression of endothelin 3 by mesenchymal cells of embryonic mouse caecum Copyright 1999 [PMID:9895385]

Expression pattern clarity: two stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:5222Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
5222_wholemount_moderate_3D_1.wlz
5222_wholemount_possible_3D_1.wlz
5222_wholemount_notDetected_3D_1.wlz
5222_wholemount_strong_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:5222_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
gut
strong strong
regionalThe only tissue displaying a strong signal at this stage was the gut in the vicinity of the developing caecum at the umbilical hernia.
forelimb bud mesenchyme
weak weak
Lower levels of reaction product can be seen associated with the mesenchyme of the developing forelimbs.
ear
weak weak
regionalweaker staining was seen in association with the otic vesicle.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:Edn3 probeA
Entity Detected:Edn3, endothelin 3 ( MGI:95285)
Sequence:sense strand is shown

>Edn3 probeA
TGAAGCTGGATCAGAGAGGGGCTGTGAAGAGACTGTGGCTGGCCCTGGTGAGAGGATTGTGTCCCCAACA
GTTGCACTGCCTGCACAGCCTGAAAGCGCTGGGCAGGAACGGGCACCAGGCAGGTCTGGGAAACAAGAGG
ACAAGGGGCTGCCTGCACACCACCGCCCTCGCCGCTGCACGTGCTTCACTTACAAGGACAAGGAGTGTGT
CTACTATTGCCACCTGGACATCATCTGGATTAACACTCCTGAACAGACTGTGCCCTATGGACTGTCCAAC
TACAGAGAAAGCCTTCGGGGAAAGAGGTCCTTGGGGCCAGTTCCAGAAAGCTCCCAGCCTTCTCCGTGGA
CACGCTTGCGTTGTACTTGTATGGGGGCGGATGACAAGGCCTGTGCACACTTCTGTGCACGCACCAGAGA
TGTCACCAGTTATTCCGGGAGAGCAGAAAGGCCAGCTGCAGAAGAGATGCGGGAGACTGGAGGCCCACGT
CAAAGGTTGATGTCAAGGACAGATAAAGCCC
nt 308 - nt 828 of NM_007903.3
Notes:The Edn3 probe used in this study by Leibl et al., 1999 [PMID:9895385] is described as "constructed using the pGEM-T vector (Promega, Southampton, UK) into which was inserted the EDN3 RT-PCR product amplified with primers (sense) 463-TGA AGC TGG ATC AGA GAG-480) and (antisense) 966-GTT CCT GTC TAT TTC GGG-983) (522 bp)."
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:CD-1
Age:10 dpc
Theiler Stage:TS18
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Leibl MA; Ota T; Woodward MN; Kenny SE; Lloyd DA; Vaillant CR; Edgar DH, 1999 [PMID:9895385] , Indexed and Spatially Mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1136/gut.44.2.246] [ PMID:9895385] Leibl MA, Ota T, Woodward MN, Kenny SE, Lloyd DA, Vaillant CR, Edgar DH 1999 Expression of endothelin 3 by mesenchymal cells of embryonic mouse caecum. Gut (44):246-52
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE