EMAGE mouse gene expression spatial database

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EMAGE:5227

Efna2 ephrin A2 ( MGI:102707)
TS17 (10.5 dpc)
in situ hybridisation

Data Images


Fig7d. Copyright: Reprinted with permission from Cell Press from [doi:10.1016/0092-8674(94)90408-1] Cell 79(1): 157-68, Cheng HJ; Flanagan JG, Identification and cloning of ELF-1, a developmentally expressed ligand for the Mek4 and Sek receptor tyrosine kinases. Copyright 1994 [PMID:7522971]

Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Notes:

Image annotations: arrowheads indicate first and second branchial arches, open triangles indicate somites, large arrow indicates midbrain, small arrow indicates hindbrain, asterisks indicate limb buds.

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

5227_wholemount_moderate_3D_1.wlz

5227_wholemount_weak_3D_1.wlz

5227_wholemount_notDetected_3D_1.wlz

5227_wholemount_strong_3D_1.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:5227_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

trunk somite

detected

ELF-l RNA expression in the somites, branchial arches, and limb buds appears mostly in a broad, diffuse pattern at this level of analysis.

branchial arch

detected

ELF-l RNA expression in the somites, branchial arches, and limb buds appears mostly in a broad, diffuse pattern at this level of analysis.

limb

detected

ELF-l RNA expression in the somites, branchial arches, and limb buds appears mostly in a broad, diffuse pattern at this level of analysis.

central nervous system

detected

regional

Includes regions of expression in the neural tube

Annotation Validation:

EMAGE Editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	Efna2 probeA
Entity Detected:	Efna2, ephrin A2 ( MGI:102707)
Sequence:	sense strand is shown >Efna2 probeA AGCGGCCTGGGTGGCTGCCACCTCTTCCTCACCACCGTCCCTGTGCTGTGGTCCCTTCTGGGCTCCTAGT GTCAGGCCGGAGAACACCAGCCCCACCTGGACCCCGTGACCTTTGCCCTCTGACCTGCCACGGCCACCTC CGAGACAAAATCCTTGCTGCTTCTCTTTCATGGTGCTGTCCCGCCGGAGGAGGCCATCCATCCGTCCCTG GGATGCAACATGGGGTCCCAATGCCTGAGGAGAAGACCCCCCCCCAAGGCTGACTCGCTTTCACCAGGCC ACCAGGGCCATCCAGTGTTGTTTAATTACAGTCGGAAAGACTTAAGGTTTTTCTTTTAATTTAATTTATT CCCTGACATTGCTGGTGACACTGGGAAGGGAACAAGCCACAGGGATGAGGTGAAGCCATCTCTGTCCTTC CTGGAATACCGGAGATCCAGGGGCCTCCAGCTGCTCCTTTCTTCTGTGTCCTGTTATTTGGGTCCCAGAT GGAGCCCACCGCGGACTTGCCTTGCATTCCTCAGGCCAGGCAAGCCTGAGCCAGAAAGGGCGCACGGTGC CAGCCCCTCTCGGGGACTCTGGGGGTGCCATCCCCCACTCTTCTTCCAGCCACTCTCGGGCCCCACTCCC ACATCATCTCAGAAACCCTTCAGCCCTCGCAACTCGCCCCTCCGGGCCCCCCCACCAGGCACAACCATCC CCGGGGCCAGCCGGGACGTTGTCGGTTTATTTCTGTAAATAGAAACCAGCAAGTGTATACTGTGATTTAT TTTAATGTATTCTTAAGGACAGAATGGAAATTCTTTAAAAAAAATTTTTTTTCCGACCTTCAATTCAAGG GGTCATTTATTTTGGTGGGGGGAGTGGGGTGGACTTTTTAGGATAGAAGCAACACTTTGCAATAAACTCA TTTTTTTTTGTTCCGTTGGAGCCCTCCCCCTTGATCATGTGACCTAGTAATGTTTATAAC
	nt 571 - nt 1540 of U14941.1
Notes:	The Efna2 (Elf-1) probe used in this study by Cheng and Flanagan, 1994 [PMID:7522971] is described as follows: "a 1kb fragment from a unique PstI site to the 3' end of clone E3-3." Editors Note: see Fig 4A therein and U14941.1 for the insert sequence of clone E3-3.
Chemistry:	RNA
Strand:	antisense

Specimen

Organism:	mouse
Age:	10.5 dpc
Theiler Stage:	TS17
Mutations:	none (wild-type)
Preparation:	wholemount

Procedures

General Information

Authors:	Cheng HJ; Flanagan JG, 1994 [PMID:7522971] , Indexed and Spatially Mapped by EMAGE.
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	non-screen
References:	[ doi:10.1016/0092-8674(94)90408-1] [ PMID:7522971] Cheng HJ, Flanagan JG 1994 Identification and cloning of ELF-1, a developmentally expressed ligand for the Mek4 and Sek receptor tyrosine kinases. Cell (79):157-68
Links:	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source