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EMAGE:5231

Efna4 ephrin A4 ( MGI:106643)
TS16 (9.5-10 dpc)
in situ hybridisation

Data Images
EMAGE:5231
Fig3I. Copyright: Reprinted with permission from Elsevier from [doi:10.1006/dbio.1996.0269] Dev Biol 179(2): 382-401, Flenniken AM; Gale NW; Yancopoulos GD; Wilkinson DG; Distinct and overlapping expression patterns of ligands for Eph-related receptor tyrosine kinases during mouse embryogenesis. Copyright 1996 [PMID:8903354]

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Image annotations: ba, branchial arch; lb, limb bud; so, somite.
Expression Pattern Description
Spatial Annotation:
EMAGE:5231Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
5231_wholemount_moderate_3D_1.wlz
5231_wholemount_weak_3D_1.wlz
5231_wholemount_notDetected_3D_1.wlz
5231_wholemount_strong_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:5231_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
trunk somite
detected detected
embryo
detected detected
regionalExpression occured in the ventral regions of the head.
limb
detected detected
branchial arch
detected detected
regionalExpression occured in the ectoderm of the branchial arches.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:Efna4 probeA
Entity Detected:Efna4, ephrin A4 ( MGI:106643)
Notes:The Efna4 (LERK4) probe used in this study by Flenniken et al., 1996 [PMID:8903354] is described as being "produced by linearization of plasmid with appropriate restriction enzymes, followed by in vitro transcription from the T7 or T3 promoters in the presence of digoxygenin-11-UTP." The probe is of unreported sequence.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Age:9.5-10 dpc
Theiler Stage:TS16
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Staining procedure:alkaline phosphatase + unspecified
General Information
Authors:Flenniken AM; Gale NW; Yancopoulos GD; Wilkinson DG, 1996 [PMID:8903354] , Indexed and Spatially Mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1006/dbio.1996.0269] [ PMID:8903354] Flenniken AM, Gale NW, Yancopoulos GD, Wilkinson DG 1996 Distinct and overlapping expression patterns of ligands for Eph-related receptor tyrosine kinases during mouse embryogenesis. Dev Biol (179):382-401
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE