Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:5232

Efna5 ephrin A5 ( MGI:107444)
TS14 (9 dpc)
in situ hybridisation

Data Images
EMAGE:5232 EMAGE:5232
Fig3D. Copyright: Reprinted with permission from Elsevier from [doi:10.1006/dbio.1996.0269] Dev Biol 179(2): 382-401, Flenniken AM; Gale NW; Yancopoulos GD; Wilkinson DG; Distinct and overlapping expression patterns of ligands for Eph-related receptor tyrosine kinases during mouse embryogenesis. Copyright 1996 [PMID:8903354] Fig4A. Copyright: Reprinted with permission from Elsevier from [doi:10.1006/dbio.1996.0269] Dev Biol 179(2): 382-401, Flenniken AM; Gale NW; Yancopoulos GD; Wilkinson DG; Distinct and overlapping expression patterns of ligands for Eph-related receptor tyrosine kinases during mouse embryogenesis. Copyright 1996 [PMID:8903354]

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Fig3D shows a lateral view of a whole-mount stained embryo, Fig4A shows a coronal cut surface through the level of the branchial arches of a whole-mount stained embryo. Image annotations: arrowheads mark apparent streams of expression; ba - branchial arch; ey - eye; fb - forebrain; mb - midbrain; ec - ectoderm.
Expression Pattern Description
Spatial Annotation:
EMAGE:5232Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
5232_wholemount_moderate_3D_1.wlz
5232_wholemount_weak_3D_1.wlz
5232_wholemount_notDetected_3D_1.wlz
5232_wholemount_strong_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:5232_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
prosencephalon
detected detected
regionalThere was a broad expression domain in the rostral forebrain.
eye
detected detected
future diencephalon
detected detected
regionalExpression was detected in a dorsal stripe.
future midbrain
detected detected
Expression was upregulated in the midbrain.
trunk somite
weak weak
future hindbrain
weak weak
1st branchial arch mandibular component ectoderm
weak weak
Intriguingly, although the expression in the branchial arches appeared to correlate with neural crest streams, in particular the absence of expression at the crest-free regions adjacent to rhombomere 3 and ventral to the otic vesicle, analysis of sections revealed AL-1/RAGS transcripts only in the ectodermal component (see Fig4A).
2nd branchial arch ectoderm
weak weak
Intriguingly, although the expression in the branchial arches appeared to correlate with neural crest streams, in particular the absence of expression at the crest-free regions adjacent to rhombomere 3 and ventral to the otic vesicle, analysis of sections revealed AL-1/RAGS transcripts only in the ectodermal component (see Fig4A).
3rd branchial arch ectoderm
weak weak
Intriguingly, although the expression in the branchial arches appeared to correlate with neural crest streams, in particular the absence of expression at the crest-free regions adjacent to rhombomere 3 and ventral to the otic vesicle, analysis of sections revealed AL-1/RAGS transcripts only in the ectodermal component (see Fig4A).
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:Efna5 probeA
Entity Detected:Efna5, ephrin A5 ( MGI:107444)
Notes:The Efna5 (AL-1/RAGS) probe used in this study by Flenniken et al., 1996 [PMID:8903354] is described as being "produced by linearization of plasmid with appropriate restriction enzymes, followed by in vitro transcription from the T7 or T3 promoters in the presence of digoxygenin-11-UTP." The probe is of unreported sequence.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Age:9 dpc
Theiler Stage:TS14
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Staining procedure:alkaline phosphatase + unspecified
General Information
Authors:Flenniken AM; Gale NW; Yancopoulos GD; Wilkinson DG, 1996 [PMID:8903354] , Indexed and Spatially Mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1006/dbio.1996.0269] [ PMID:8903354] Flenniken AM, Gale NW, Yancopoulos GD, Wilkinson DG 1996 Distinct and overlapping expression patterns of ligands for Eph-related receptor tyrosine kinases during mouse embryogenesis. Dev Biol (179):382-401
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE