Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:5233

Efna5 ephrin A5 ( MGI:107444)
TS15 (9.5 dpc)
in situ hybridisation

Data Images
EMAGE:5233
Fig7A. from [doi:10.1093/emboj/cdg169] Trokovic R; Trokovic N; Hernesniemi S; Pirvola U; Vogt Weisenhorn DM; Rossant J; McMahon AP; Wurst W; Partanen J. EMBO J 2003 22(8): 1811-1823. FGFR1 is independently required in both developing mid- and hindbrain for sustained response to isthmic signals. [PMID:12682014] © Nature Publishing Group. Reproduced with permission from EMBO J.

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Image annotation: arrowhead indicates the midbrain-hindbrain boundary.
Expression Pattern Description
Spatial Annotation:
EMAGE:5233Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
5233_wholemount__moderate.wlz
5233_wholemount__possible.wlz
5233_wholemount__notDetected.wlz
5233_wholemount__strong.wlz
(what is wlz format?)
Download all expression domains: EMAGE:5233_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
future midbrain
detected detected
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:Efna5 probeB
Entity Detected:Efna5, ephrin A5 ( MGI:107444)
Notes:The Efna5 probe used in this study by Trokovic et al., 2003 [PMID:12682014] is described as "a gift from David Wilkinson", and is of unreported sequence.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Age:9.5 dpc
Theiler Stage:TS15
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
Staining procedure:alkaline phosphatase + unspecified
General Information
Authors:Trokovic R; Trokovic N; Hernesniemi S; Pirvola U; Vogt Weisenhorn DM; Rossant J; McMahon AP; Wurst W; Partanen J, 2003 [PMID:12682014] , Indexed and Spatially Mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1093/emboj/cdg169] [ PMID:12682014] Trokovic R, Trokovic N, Hernesniemi S, Pirvola U, Vogt Weisenhorn DM, Rossant J, McMahon AP, Wurst W, Partanen J 2003 FGFR1 is independently required in both developing mid- and hindbrain for sustained response to isthmic signals. EMBO J (22):1811-23
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE