EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:5234

Efnb1 ephrin B1 ( MGI:102708)
TS18 (10.5 dpc)
in situ hybridisation

Data Images


Fig 4B. [doi:10.1073/pnas.0402819101] Twigg SR; Kan R; Babbs C; Bochukova EG; Robertson SP; Wall SA; Morriss-Kay GM; Wilkie AO, Proc Natl Acad Sci U S A 2004 Jun 8;101(22):8652-7. [PMID:15166289] Copyright 2004 National Academy of Sciences, U.S.A.	Fig 4C. [doi:10.1073/pnas.0402819101] Twigg SR; Kan R; Babbs C; Bochukova EG; Robertson SP; Wall SA; Morriss-Kay GM; Wilkie AO, Proc Natl Acad Sci U S A 2004 Jun 8;101(22):8652-7. [PMID:15166289] Copyright 2004 National Academy of Sciences, U.S.A.

Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Notes:

(b) wholemount E10.5 embryo. Arrow marks the boundary between a high level of expression in the region of the telencephalon compared with the adjacent diencephalon; t - telencephalon; e - eye. (c) Section of an E10.5 embryo showing high Efnb1 transcript levels in the neuroepithelium (nep) of the telencephalon and adjacent neural crest-derived mesenchyme (nc) but not in the diencephalon (d) or cranial mesoderm (m). Arrow shows the boundary between neural crest-derived and mesoderm-derived cranial mesenchyme, which marks the position of the future coronal suture.

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

5234_wholemount_moderate_3D_1.wlz

5234_wholemount_weak_3D_1.wlz

5234_wholemount_notDetected_3D_1.wlz

5234_wholemount_strong_3D_1.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:5234_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

head mesenchyme derived from neural crest

detected

Efnb1 expression domain corresponds specifically to the neural crest-derived, future frontal bone territory, but is absent from the mesenchyme that is destined to form the parietal bone.

telencephalon

detected

regional

Efnb1 expression domain corresponds specifically to the telencephalic vesicle neuroepithelium (which underlies the neural crest-derived, future frontal bone territory where Efnb1 is also expressed).

Annotation Validation:

EMAGE Editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	Efnb1 probeB
Entity Detected:	Efnb1, ephrin B1 ( MGI:102708)
Notes:	The Efnb1 (Elk-L) probe used in this study by Twigg et al., 2004 [PMID:15166289] is indicated as that previously described by Flenniken et al., 1996 [PMID:8903354] , which was "produced by linearization of plasmid with appropriate restriction enzymes, followed by in vitro transcription from the T7 or T3 promoters in the presence of digoxygenin-11-UTP." The probe is of unreported sequence.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Age:	10.5 dpc
Theiler Stage:	TS18
Mutations:	none (wild-type)
Preparation:	wholemount

Procedures

Staining procedure:

alkaline phosphatase + unspecified

General Information

Authors:	Twigg SR; Kan R; Babbs C; Bochukova EG; Robertson SP; Wall SA; Morriss-Kay GM; Wilkie AO, 2004 [PMID:15166289] , Indexed and Spatially Mapped by EMAGE.
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	non-screen
References:	[ doi:10.1073/pnas.0402819101] [ PMID:15166289] Twigg SR, Kan R, Babbs C, Bochukova EG, Robertson SP, Wall SA, Morriss-Kay GM, Wilkie AO 2004 Mutations of ephrin-B1 (EFNB1), a marker of tissue boundary formation, cause craniofrontonasal syndrome. Proc Natl Acad Sci U S A (101):8652-7
	[ doi:10.1006/dbio.1996.0269] [ PMID:8903354] Flenniken AM, Gale NW, Yancopoulos GD, Wilkinson DG 1996 Distinct and overlapping expression patterns of ligands for Eph-related receptor tyrosine kinases during mouse embryogenesis. Dev Biol (179):382-401
Links:	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source