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EMAGE:5235

Efnb2 ephrin B2 ( MGI:105097)
TS16 (9.5 dpc)
in situ hybridisation

Data Images
EMAGE:5235
Fig6C. Copyright: Reprinted with permission from Elsevier from [doi:10.1006/dbio.2000.9969] Dev Biol 229(1): 176-87, Johnson J; Rhee J; Parsons SM; Brown D; Olson EN; Rawls A, The anterior/posterior polarity of somites is disrupted in paraxis-deficient mice. Copyright 2001 [PMID:11133162]

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Image annotations: red arrowheads - posterior-restricted transcription of ephrin-B2 in the somites.
Expression Pattern Description
Spatial Annotation:
EMAGE:5235Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
5235_wholemount__moderate.wlz
5235_wholemount__weak.wlz
5235_wholemount__notDetected.wlz
5235_wholemount__strong.wlz
(what is wlz format?)
Download all expression domains: EMAGE:5235_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
trunk somite
detected detected
regionalPosterior-restricted transcription of ephrin-B2 was noted in the somites of wild-type embryos.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:Efnb2 probeB
Entity Detected:Efnb2, ephrin B2 ( MGI:105097)
Notes:The Efnb2 (ephrin B2/ELF-2) probe used in this study by Johnson et al., 2001 [PMID:11133162] is indicated as that previously described by Bergemann et al., 1995 [PMID:7651410] i.e. as one of three possible probes which all were reported to give the same expression pattern. The probes are described as follows, "One, pELF2E, is a subclone of E2.2 containing coding sequence extendingfrom the 59 end of clone E2.2 at nucleotide 262 to a PstI site at nucleotide 895. Two more subclones, pELF2H and pELF2I, were generated from the 3' ends of clones E2.1 and E2.3. These clones contain inserts of 700 and 500 bp, respectively, from the 3' untranslated region of the cDNA. All three subclones were made in the pBluescript SK vector and were cut at sites in the polylinker and used as templates for T3 and T7 RNA polymerases to produce sense and antisense probes for ELF-2 RNA."
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Age:9.5 dpc
Theiler Stage:TS16
Mutations:none (wild-type)
Preparation:wholemount
Procedures
General Information
Authors:Johnson J; Rhee J; Parsons SM; Brown D; Olson EN; Rawls A, 2001 [PMID:11133162] , Indexed and Spatially Mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1006/dbio.2000.9969] [ PMID:11133162] Johnson J, Rhee J, Parsons SM, Brown D, Olson EN, Rawls A 2001 The anterior/posterior polarity of somites is disrupted in paraxis-deficient mice. Dev Biol (229):176-87
 [ PMID:7651410] Bergemann AD, Cheng HJ, Brambilla R, Klein R, Flanagan JG 1995 ELF-2, a new member of the Eph ligand family, is segmentally expressed in mouse embryos in the region of the hindbrain and newly forming somites. Mol Cell Biol (15):4921-9
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE