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EMAGE:5238

Efnb1 ephrin B1 ( MGI:102708)
TS15 (9.5 dpc)
in situ hybridisation

Data Images
EMAGE:5238
Fig 4A. [doi:10.1073/pnas.0402819101] Twigg SR; Kan R; Babbs C; Bochukova EG; Robertson SP; Wall SA; Morriss-Kay GM; Wilkie AO, Proc Natl Acad Sci U S A 2004 Jun 8;101(22):8652-7. [PMID:15166289] Copyright 2004 National Academy of Sciences, U.S.A.

Expression pattern clarity: two stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Image annotations: e - eye.
Expression Pattern Description
Spatial Annotation:
EMAGE:5238Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
5238_wholemount__moderate.wlz
5238_wholemount__notDetected.wlz
5238_wholemount__strong.wlz
5238_wholemount__possible.wlz
(what is wlz format?)
Download all expression domains: EMAGE:5238_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
head mesenchyme derived from neural crest
detected detected
Efnb1 expression domain corresponds specifically to the neural crest-derived, future frontal bone territory, but is absent from the mesenchyme that is destined to form the parietal bone.
telencephalic vesicle
detected detected
regionalEfnb1 expression domain corresponds specifically to the telencephalic vesicle neuroepithelium.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:Efnb1 probeB
Entity Detected:Efnb1, ephrin B1 ( MGI:102708)
Notes:The Efnb1 (Elk-L) probe used in this study by Twigg et al., 2004 [PMID:15166289] is indicated as that previously described by Flenniken et al., 1996 [PMID:8903354] , which was "produced by linearization of plasmid with appropriate restriction enzymes, followed by in vitro transcription from the T7 or T3 promoters in the presence of digoxygenin-11-UTP." The probe is of unreported sequence.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Age:9.5 dpc
Theiler Stage:TS15
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Staining procedure:alkaline phosphatase + unspecified
General Information
Authors:Twigg SR; Kan R; Babbs C; Bochukova EG; Robertson SP; Wall SA; Morriss-Kay GM; Wilkie AO, 2004 [PMID:15166289] , Indexed and Spatially Mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1073/pnas.0402819101] [ PMID:15166289] Twigg SR, Kan R, Babbs C, Bochukova EG, Robertson SP, Wall SA, Morriss-Kay GM, Wilkie AO 2004 Mutations of ephrin-B1 (EFNB1), a marker of tissue boundary formation, cause craniofrontonasal syndrome. Proc Natl Acad Sci U S A (101):8652-7
 [ doi:10.1006/dbio.1996.0269] [ PMID:8903354] Flenniken AM, Gale NW, Yancopoulos GD, Wilkinson DG 1996 Distinct and overlapping expression patterns of ligands for Eph-related receptor tyrosine kinases during mouse embryogenesis. Dev Biol (179):382-401
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE