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EMAGE:5242

Eif2c1 eukaryotic translation initiation factor 2C, 1 ( MGI:2446630)
TS19 (11.5 dpc)
in situ hybridisation

Data Images
EMAGE:5242
Fig1A. [doi:10.1016/j.bbrc.2005.05.206] Reprinted from Biochem Biophys Res Comm, vol 334, Lu J; Qian J; Chen F; Tang X; Li C; Cardoso WV, Differential expression of components of the microRNA machinery during mouse organogenesis., pp 319-23, Copyright (2005) with permission from Elsevier. [PMID:16036130]

Expression pattern clarity: two stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Image annotations: neural tube (nt), dorsal root ganglia (drg), and somites (so), forebrain (fb), midbrain (mb), Arrowheads and asterisks depict the presence or absence of signals, respectively.
Expression Pattern Description
Spatial Annotation:
EMAGE:5242Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
5242_wholemount_moderate_3D_1.wlz
5242_wholemount_weak_3D_1.wlz
5242_wholemount_notDetected_3D_1.wlz
5242_wholemount_strong_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:5242_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
neural tube
strong strong
Ago1 was present in the medial column and dorsal root ganglia.
dorsal root ganglion
detected detected
forebrain
strong strong
regionalAgo1 is also strongly expressed in the neuroepithelium of the forebrain and midbrain.
midbrain
strong strong
regionalAgo1 is also strongly expressed in the neuroepithelium of the forebrain and midbrain.
limb
detected detected
trunk somite
detected detected
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:3587199
Entity Detected:Eif2c1, eukaryotic translation initiation factor 2C, 1 ( MGI:2446630)
Sequence:sense strand is shown

>MGI:3587199
GCCATTCGAGATGCATGCATCAAACTGGAAAAAGACTACCAGCCTGGGATCACATATATTGTGGTGCAGA
AGCGCCATCACACCCGCCTGTTCTGTGCTGACAAGAATGAGCGGATTGGGAAGAGTGGTAACATCCCAGC
AGGGACCACTGTGGACACCAACATCACCCACCCATTTGAGTTCGACTTCTATCTGTGCAGCCATGCAGGC
ATCCAGGGTACCAGCCGACCATCCCATTATTATGTCCTTTGGGATGACAACCGTTTCACAGCAGATGAAC
TCCAGATCTTGACATACCAGCTGTGCCACACTTACGTACGATGCACACGTTCTGTCTCTATCCCAGCACC
TGCCTACTATGCTCGCTTGGTGGCTTTCCGGGCACGATACCACCTGGTGGACAAGGAACATGACAGTGGA
GAGGGGAGCCACATATCAGGGCAAAGCAATGG
nt 2050 - nt 2501 of NM_153403.1
Notes:The Eif2c1 (Ago1) probe template used in this study by Lu et al., 2005 [PMID:16036130] was "generated by PCR, using GenBank sequences as described: Ago1 NM_153403. 5'primer: AATTAACCCTCACTAAAGGGGCCATTCGAGATGCATGCAT 3'primer: TAATACGACTCACTATAGGGCCATTGCTTTGCCCTGATAT"
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Age:11.5 dpc
Theiler Stage:TS19
Mutations:none (wild-type)
Preparation:wholemount
Procedures
General Information
Authors:Lu J; Qian J; Chen F; Tang X; Li C; Cardoso WV, 2005 [PMID:16036130] , Indexed by GXD, Spatially Mapped by EMAGE
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1016/j.bbrc.2005.05.206] [ PMID:16036130] Lu J, Qian J, Chen F, Tang X, Li C, Cardoso WV 2005 Differential expression of components of the microRNA machinery during mouse organogenesis. Biochem Biophys Res Commun (2005):319-23
Links:MGI:3587194 primer sequences
 MGI:3587207 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI