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EMAGE:5244

Eif2c3 eukaryotic translation initiation factor 2C, 3 ( MGI:2446634)
TS19 (11.5 dpc)
in situ hybridisation

Data Images
EMAGE:5244
Fig1E. [doi:10.1016/j.bbrc.2005.05.206] Reprinted from Biochem Biophys Res Comm, vol 334, Lu J; Qian J; Chen F; Tang X; Li C; Cardoso WV, Differential expression of components of the microRNA machinery during mouse organogenesis., pp 319-23, Copyright (2005) with permission from Elsevier. [PMID:16036130]

Expression pattern clarity: two stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Image annotations: neural tube (nt), dorsal root ganglia (drg), and somites (so), forebrain (fb), midbrain (mb), Arrowheads and asterisks depict the presence or absence of signals, respectively.
Expression Pattern Description
Spatial Annotation:
EMAGE:5244Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
5244_wholemount_moderate_3D_1.wlz
5244_wholemount_weak_3D_1.wlz
5244_wholemount_notDetected_3D_1.wlz
5244_wholemount_strong_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:5244_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
neural tube
weak weak
limb
detected detected
trunk somite
detected detected
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:3587201
Entity Detected:Eif2c3, eukaryotic translation initiation factor 2C, 3 ( MGI:2446634)
Sequence:sense strand is shown

>MGI:3587201
GTCCTTCACACTATCATGTTTTATGGGATGACAACTTCTTTACTGCAGATGAACTTCAGCTGCTCACTTA
CCAGCTCTGTCACACTTACGTACGCTGTACAAGATCTGTTTCTATCCCTGCACCAGCATATTACGCTCAC
CTGGTGGCATTCAGAGCCAGATATCATCTTGTGGACAAGGAACATGACAGTGCTGAAGGAAGCCATGTTT
CAGGACAGAGCAATGGGCGAGATCCACAAGCTCTTGCCAAGGCTGTACAGATTCACCAAGATACCTTACG
CACAATGTACTTCGCTTAACAAGTCCAAGTTTATTCTCCGTGACAA
nt 2393 - nt 2718 of NM_153402.1
Notes:The Eif2c3 (Ago3) probe used in this study by Lu et al., 2005 [PMID:16036130] is described as being "generated by PCR, using GenBank sequence Ago3, NM_153402. 5' primer: AATTAACCCTCACTAAAGGGGTCCTTCACACTATCATGTT 3' primer: TAATACGACTCACTATAGGGTTGTCACGGAGAATAAACTT."
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Age:11.5 dpc
Theiler Stage:TS19
Mutations:none (wild-type)
Preparation:wholemount
Procedures
General Information
Authors:Lu J; Qian J; Chen F; Tang X; Li C; Cardoso WV, 2005 [PMID:16036130] , Indexed by GXD, Spatially Mapped by EMAGE
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1016/j.bbrc.2005.05.206] [ PMID:16036130] Lu J, Qian J, Chen F, Tang X, Li C, Cardoso WV 2005 Differential expression of components of the microRNA machinery during mouse organogenesis. Biochem Biophys Res Commun (2005):319-23
Links:MGI:3587196 primer sequences
 MGI:3587209 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI