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EMAGE:5245

Eif2c4 eukaryotic translation initiation factor 2C, 4 ( MGI:1924100)
TS19 (11.5 dpc)
in situ hybridisation

Data Images
EMAGE:5245
Fig1G. [doi:10.1016/j.bbrc.2005.05.206] Reprinted from Biochem Biophys Res Comm, vol 334, Lu J; Qian J; Chen F; Tang X; Li C; Cardoso WV, Differential expression of components of the microRNA machinery during mouse organogenesis., pp 319-23, Copyright (2005) with permission from Elsevier. [PMID:16036130]

Expression pattern clarity: two stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Image annotations: neural tube (nt), dorsal root ganglia (drg), and somites (so), forebrain (fb), midbrain (mb), Arrowheads and asterisks depict the presence or absence of signals, respectively.
Expression Pattern Description
Spatial Annotation:
EMAGE:5245Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
5245_wholemount_moderate_3D_1.wlz
5245_wholemount_weak_3D_1.wlz
5245_wholemount_notDetected_3D_1.wlz
5245_wholemount_strong_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:5245_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
neural tube
strong strong
regionalExpression is present in the lateral column of neurons.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:3587202
Entity Detected:Eif2c4, eukaryotic translation initiation factor 2C, 4 ( MGI:1924100)
Sequence:sense strand is shown

>MGI:3587202
CCTCTGTAGTCATGCAGGAATTCAGGGAACCAGCCGTCCTTCACATTACCAGGTCTTGTGGGATGACAAC
TGCTTCACTGCAGATGAACTCCAGCTACTGACTTACCAGCTCTGTCACACCTACGTGAGGTGCACACGCT
CCGTCTCCATTCCTGCCCCTGCATATTATGCCCGGCTTGTGGCATTCCGGGCAAGGTATCATTTGGTGGA
TAAAGATCATGACAGTGCGGAAGGCAGTCACGTGTCAGGACAGAGCAACGGCCGGGATCCTCAGGCCTTG
GCTAAGGCTGTGCAGATCCACCATGATACCCAGCACACTATGTATTTTGCCTGAGAGTCTTGGAAAAGAA
CTCAACAAATTTGGCATCTCACGCATCCTC
nt 2290 - nt 2669 of NM_153177.2
Notes:The Eif2c4 (Ago4) probe used in this study by Lu et al., 2005 [PMID:16036130] is described as being generated by PCR, using GenBank sequence, NM_153177, 5' primer: AATTAACCCTCACTAAAGGGCCTCTGTAGTCATGCAGGAA and 3' primer: TAATACGACTCACTATAGGGGAGGATACGTGAGATGCCAA.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Age:11.5 dpc
Theiler Stage:TS19
Mutations:none (wild-type)
Preparation:wholemount
Procedures
General Information
Authors:Lu J; Qian J; Chen F; Tang X; Li C; Cardoso WV, 2005 [PMID:16036130] , Indexed by GXD, Spatially Mapped by EMAGE
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1016/j.bbrc.2005.05.206] [ PMID:16036130] Lu J, Qian J, Chen F, Tang X, Li C, Cardoso WV 2005 Differential expression of components of the microRNA machinery during mouse organogenesis. Biochem Biophys Res Commun (2005):319-23
Links:MGI:3587197 primer sequences
 MGI:3587210 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE