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EMAGE:5250

Enah enabled homolog (Drosophila) ( MGI:108360)
TS17 (10.5 dpc)
in situ hybridisation

Data Images
EMAGE:5250 EMAGE:5250
Fig3D. Copyright: Reprinted with permission from Cell Press from [doi:10.1016/S0896-6273(00)81092-2] Neuron 22(2): 313-25, Lanier LM; Gates MA; Witke W; Menzies AS; Wehman AM; Macklis JD; Kwiatkowski D; Soriano P; Gertler FB, Mena is required for neurulation and commissure formation. Copyright 1999. [PMID:10069337] Fig3C. Copyright: Reprinted with permission from Cell Press from [doi:10.1016/S0896-6273(00)81092-2] Neuron 22(2): 313-25, Lanier LM; Gates MA; Witke W; Menzies AS; Wehman AM; Macklis JD; Kwiatkowski D; Soriano P; Gertler FB, Mena is required for neurulation and commissure formation. Copyright 1999. [PMID:10069337]

Expression pattern clarity: two stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
(C) and (D) show the same embryo. Image annotations: forebrain (fb), midbrain (mb), branchial arch (ba), pharyngeal arch (pa), forelimb bud (flb), hindlimb bud (hlb), somites (S), dorsal root ganglia (DRG).
Expression Pattern Description
Spatial Annotation:
EMAGE:5250Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
5250_wholemount__moderate.wlz
5250_wholemount__weak.wlz
5250_wholemount__notDetected.wlz
5250_wholemount__strong.wlz
(what is wlz format?)
Download all expression domains: EMAGE:5250_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
dorsal root ganglion
detected detected
trunk somite
detected detected
limb
detected detected
branchial arch
strong strong
Mena was highly expressed in the branchial and pharyngeal arches.
head mesenchyme derived from neural crest
strong strong
Mena was highly expressed in the neural crest-derived structures that give rise to portions of the face and neck.
forebrain
detected detected
midbrain
detected detected
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:Enah probeA
Entity Detected:Enah, enabled homolog (Drosophila) ( MGI:108360)
Notes:The Enah (Mena) probe used in this study by Lanier et al., 1999 [PMID:10069337] is described as being "prepared by in vitro transcription of linearized template DNA" (covering the entire Mena coding sequence). Editors Note: this probe detects all Mena transcripts. The authors also used a probe specific to the Mena(+) exon (the results of which are shown in Fig 3E).
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Age:10.5 dpc
Theiler Stage:TS17
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Lanier LM; Gates MA; Witke W; Menzies AS; Wehman AM; Macklis JD; Kwiatkowski D; Soriano P; Gertler FB, 1999 [PMID:10069337] , Indexed and Spatially Mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1016/S0896-6273(00)81092-2] [ PMID:10069337] Lanier LM, Gates MA, Witke W, Menzies AS, Wehman AM, Macklis JD, Kwiatkowski D, Soriano P, Gertler FB 1999 Mena is required for neurulation and commissure formation. Neuron (22):313-25
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE