EMAGE mouse gene expression spatial database

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EMAGE:5251

Enc1 ectodermal-neural cortex 1 ( MGI:109610)
TS14 (9 dpc)
in situ hybridisation

Data Images


Fig5D. Copyright: Reprinted with permission from the Society for Neuroscience from J Neurosci 17(9): 3038-51. Hernandez MC; Andres-Barquin PJ; Martinez S; Bulfone A; Rubenstein JL ; Israel MA. ENC-1: a novel mammalian kelch-related gene specifically expressed in the nervous system encodes an actin-binding protein. Copyright 1997. [PMID:9096139]

Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Notes:

Image annotations: telencephalic vesicle (T), midbrain (MB), prorhombomere A (PRA), mandibular process (MD), and caudal neuropore (CN). The open arrows show the neural crest cells that are labeled in their migration pathway to the branchial arches and body segments.

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

5251_wholemount__moderate.wlz

5251_wholemount__weak.wlz

5251_wholemount__notDetected.wlz

5251_wholemount__strong.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:5251_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

prosencephalon

detected

regional

expression was detected in the neuroepithelium of the caudal pole of the telencephalic vesicle.

head mesenchyme derived from neural crest

detected

Mesenchymal cells (probably neural crest cells) in the head and body are labeled.

trunk mesenchyme derived from neural crest

detected

Mesenchymal cells (probably neural crest cells) in the head and body are labeled.

caudal neuropore

detected

Caudally, ENC-1 was expressed in the margins of the caudal neuropore.

rhombomere 01

detected

RNA expression was detected in the rostral rhombencephalon (pro-rhombomere A). Editors Note: Pro-rhombomere A develops into rhombomeres 1 and 2 around this time.

rhombomere 02

detected

RNA expression was detected in the rostral rhombencephalon (pro-rhombomere A). Editors Note: Pro-rhombomere A develops into rhombomeres 1 and 2 around this time.

neural tube

detected

regional

RNA expression was detected in the alar plate of the spinal cord.

Annotation Validation:

EMAGE Editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	Enc1 probeA
Entity Detected:	Enc1, ectodermal-neural cortex 1 ( MGI:109610)
Sequence:	sense strand is shown >Enc1 probeA CGCACGGTCACTCTCCCCTGGCTAGGCGCGCGCCGGCAGACGCTTGGCCCGGCCGGGGCGCCAGGTCCGT AGCAGCAGATCCGGAGACCGAGGTCAGCTGCCTGCCGAAACCAGCCCGGGAGCCACTACCCCGGACGCTA TCGCCTCCCTTCCCAGCGCTCCTTTCTCCCCGGCGATCGCCACCGCTTCCTGGAACAGAGACGCCCTTCG TCGGCTCCCTGCGGTGACCGAGGCAAGAACCGCAGCCGGTTGTGTGCAGAGGCCAGCCGCCACTACTGAG TTGAAACAAAATGTCAGTCAGTGTGCATGAGAACCGCAAGTCCAGGGCCAGCAGTGGCTCCATCAACATC TACCTGTTTCATAAGTCCTCCTACGCGGACAGCGTTCTCACTCACCTGAACCTTCTGCGTCAGCAGCGGC TCTTCACAGATGTCCTTCTCCATGCGGGAAACAGGACCTTCCCTTGCCACCGGGCAGTGCTGGCTGCGTG CAGCCGCTACTTCGAAGCCATGTTCAGTGGTGGCCTGAAAGAGAGCCAGGACAGTGAGGTGAACTTCGAC AATTCCATCCACCCAGAAGTCTTAGAGCTGCTTCTAGACTACGCATACTCCTCCCGGGTCATTATCAATG AAGAAAATGCTGAGTCGCTCCTGGAGGCTGGTGACATGCTGGAGTTCCAGGACATCAGAGATGCGTGTGC AGAATTTCTAGAGAAGAACCTGCATCCCACCAACTGCCTGGGTATGCTGCTGTTGTCTGATGCCCACCAG TGCACCAAGCTGTACGAACTCTCCTGGAGAATGTGTCTCAGCAACTTCCAAACCATTCGGAAGAGCGAAG ATTTCCTCCAGTTGCCCCAGGACATGGTTGTGCAGCTGCTGTCCAGTGAAGAACTGGAGACGGAAGACGA AAGGCTGGTGTATGAGTCTGCGATGAACTGGATCAGCTATGACCTGAAGAAGCGCTACTGTTACCTCCCG GAACTGTTGCAGACAGTGAGGCTGGCCCTCCTTCCTGCCATCTATCTCATGGAGAACGTGGCGATGGAAG AACTCATCACCAAGCAGAGAAAGAGTAAGGAGATCGTGGAAGAGGCCATCAGGTGCAAACTAAAAATCTT ACAGAATGACGGCGTGGTCACCAGTCTCTGTGCTCGTCCTCGGAAAACTGGCCATGCCCTGTTCCTCCTG GGAGGGCAGACTTTCATGTGTGACAAACTGTACTTGGTAGACCAGAAGGCTAAAGAAATCATTCCCAAGG CTGACATTCCCAGCCCGAGGAAAGAGTTCAGCGCATGTGCAATTGGCTGCAAAGTATATATTACTGGGGG GCGGGGATCAGAGAACGGAGTCTCAAAAGATGTCTGGGTTTACGATACCCTGCATGAGGAGTGGTCCAAG GCTGCCCCCATGCTGGTGGCCAGGTTTGGCCATGGATCTGCTGAACTGAAGCACTGCCTCTATGTAGTCG GTGGGCACACAGCTGCAACTGGCTGCCTCCCAGCCTCCCCCTCGGTCTCCCTAAAGCAAGTAGAACAGTA TGACCCCACAACCAACAAATGGACCATGGTAGCCCCACTCCGCGAAGGTGTCAGCAATGCTGCTGTAGTG AGTGCCAAACTTAAGCTGTTTGCTTTCGGGGGTACCAGTGTGAGCCACGACAAGCTGCCCAAGGTTCAGT GTTACGATCAATGCGAGAACAGATGGTCAGTGCCGGCCACCTGTCCCCAGCCCTGGCGTTACACAGCCGC AGCTGTGCTGGGAAACCAGATTTTTATCATGGGTGGAGATACAGAGTTCTCTGCCTGCTCCGCTTACAAA TTCAATAGTGAGACTTACCAGTGGACCAAGGTGGGAGATGTGACAGCCAAGCGCATGAGCTGCCACGCCG TGGCCTCCGGGAACAAGCTTTACGTAGTTGGAGGGTACTTCGGCATTCAGCGCTGCAAGACTTTGGACTG TTACGACCCAACTTTAGATGTGTGGAACAGCATAACCACTGTTCCCTACTCTCTGATCCCTACTGCATTC GTCAGCACCTGGAAACACCTGCCTTCCTAATGTAGAGCATCCTAAAGAAAGCACGCATGAGCTCATTCTG ATGCACGGCGAGATGAGATGTCATTTCTGCTTTGGAGAAGGCAAGTTTAATGAAGAGAAAGAAAAACAGG AGCAGTTGCTACTGAGACTCCTCGAATACCATCGGCTGCACCTTGCAAACACTCTCAAGTGGACATGAAG GAAGGGGTGGGGGGAGGGGCGGGTTTTTTTTTTTTTCAATATAAGTAGCACATGGTTTAA
	nt 1 - nt 2300 of U65079.1
Notes:	The Enc1 probe used in this study by Hernandez et al., 1997 [PMID:9096139] is described as follows: "the antisense ENC-1 riboprobe was synthesized using a digoxigenin RNA labeling kit (Boehringer Mannheim) and DNA from the clone p10.2x." Editors Note: see Fig2 therein and U65079.1 for the insert sequence of clone p10.2x. Note that U65079.1 does not include the long poly(A) tail shown in Fig2.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Age:	9 dpc
Theiler Stage:	TS14
Mutations:	none (wild-type)
Preparation:	wholemount

Procedures

Staining procedure:

alkaline phosphatase + unspecified

General Information

Authors:	Hernandez MC; Andres-Barquin PJ; Martinez S; Bulfone A; Rubenstein JL; Israel MA, 1997 [PMID:9096139] , Indexed and Spatially Mapped by EMAGE.
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	non-screen
References:	[ PMID:9096139] Hernandez MC, Andres-Barquin PJ, Martinez S, Bulfone A, Rubenstein JL, Israel MA 1997 ENC-1: a novel mammalian kelch-related gene specifically expressed in the nervous system encodes an actin-binding protein. J Neurosci (17):3038-51
Links:	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source