EMAGE mouse gene expression spatial database

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EMAGE:5253

Enc1 ectodermal-neural cortex 1 ( MGI:109610)
TS17 (10.5 dpc)
in situ hybridisation

Data Images


Fig5J. Copyright: Reprinted with permission from the Society for Neuroscience from J Neurosci 17(9): 3038-51. Hernandez MC; Andres-Barquin PJ; Martinez S; Bulfone A; Rubenstein JL ; Israel MA. ENC-1: a novel mammalian kelch-related gene specifically expressed in the nervous system encodes an actin-binding protein. Copyright 1997. [PMID:9096139]

Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Notes:

Image annotations: The thin arrow in j shows the boundary between midbrain (MB) and diencephalon (D). The asterisk indicates that some labeling is apparent in the prosencephalic basal plate. The open arrows indicate the somitomeres that express ENC-1, which at this stage appears in the tail bud. MX - Maxillary process; MD - Mandibular process; CB - Cerebellar plate; P1-P6 - Prosomeres 1-6; OV - Optic Vesicle; T - telencephalon.

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

5253_wholemount__moderate.wlz

5253_wholemount__weak.wlz

5253_wholemount__notDetected.wlz

5253_wholemount__strong.wlz

5253_wholemount__possible.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:5253_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

telencephalon

detected

graded

ENC-1 expression was observed in the Telencephalon, where the cortical anlage showed more intense labeling than the basal ganglia anlage.

midbrain

detected

graded

The mesencephalon (MB) showed a rostro-caudal gradient of expression that started at the mesencephalic-diencephalic boundary.

hindbrain

detected

regional

ENC-1 iso expressed in dorsal areas of the rhombencephalon.

future spinal cord

detected

regional

ENC-1 is expressed in dorsal areas of the spinal cord.

dorsal root ganglion

detected

regional

ENC-1 expression was also localized to the neural crest-derived cells of the dorsal root ganglia.

diencephalon

weak

regional

weak expression of ENC-1 appeared in the basal plate of prosomeres1 and 2. The caudal diencephalon showed scattered cells expressing ENC-1 in ventral areas of the alar plate of prosomere 1 (P1).

Annotation Validation:

EMAGE Editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	Enc1 probeA
Entity Detected:	Enc1, ectodermal-neural cortex 1 ( MGI:109610)
Sequence:	sense strand is shown >Enc1 probeA CGCACGGTCACTCTCCCCTGGCTAGGCGCGCGCCGGCAGACGCTTGGCCCGGCCGGGGCGCCAGGTCCGT AGCAGCAGATCCGGAGACCGAGGTCAGCTGCCTGCCGAAACCAGCCCGGGAGCCACTACCCCGGACGCTA TCGCCTCCCTTCCCAGCGCTCCTTTCTCCCCGGCGATCGCCACCGCTTCCTGGAACAGAGACGCCCTTCG TCGGCTCCCTGCGGTGACCGAGGCAAGAACCGCAGCCGGTTGTGTGCAGAGGCCAGCCGCCACTACTGAG TTGAAACAAAATGTCAGTCAGTGTGCATGAGAACCGCAAGTCCAGGGCCAGCAGTGGCTCCATCAACATC TACCTGTTTCATAAGTCCTCCTACGCGGACAGCGTTCTCACTCACCTGAACCTTCTGCGTCAGCAGCGGC TCTTCACAGATGTCCTTCTCCATGCGGGAAACAGGACCTTCCCTTGCCACCGGGCAGTGCTGGCTGCGTG CAGCCGCTACTTCGAAGCCATGTTCAGTGGTGGCCTGAAAGAGAGCCAGGACAGTGAGGTGAACTTCGAC AATTCCATCCACCCAGAAGTCTTAGAGCTGCTTCTAGACTACGCATACTCCTCCCGGGTCATTATCAATG AAGAAAATGCTGAGTCGCTCCTGGAGGCTGGTGACATGCTGGAGTTCCAGGACATCAGAGATGCGTGTGC AGAATTTCTAGAGAAGAACCTGCATCCCACCAACTGCCTGGGTATGCTGCTGTTGTCTGATGCCCACCAG TGCACCAAGCTGTACGAACTCTCCTGGAGAATGTGTCTCAGCAACTTCCAAACCATTCGGAAGAGCGAAG ATTTCCTCCAGTTGCCCCAGGACATGGTTGTGCAGCTGCTGTCCAGTGAAGAACTGGAGACGGAAGACGA AAGGCTGGTGTATGAGTCTGCGATGAACTGGATCAGCTATGACCTGAAGAAGCGCTACTGTTACCTCCCG GAACTGTTGCAGACAGTGAGGCTGGCCCTCCTTCCTGCCATCTATCTCATGGAGAACGTGGCGATGGAAG AACTCATCACCAAGCAGAGAAAGAGTAAGGAGATCGTGGAAGAGGCCATCAGGTGCAAACTAAAAATCTT ACAGAATGACGGCGTGGTCACCAGTCTCTGTGCTCGTCCTCGGAAAACTGGCCATGCCCTGTTCCTCCTG GGAGGGCAGACTTTCATGTGTGACAAACTGTACTTGGTAGACCAGAAGGCTAAAGAAATCATTCCCAAGG CTGACATTCCCAGCCCGAGGAAAGAGTTCAGCGCATGTGCAATTGGCTGCAAAGTATATATTACTGGGGG GCGGGGATCAGAGAACGGAGTCTCAAAAGATGTCTGGGTTTACGATACCCTGCATGAGGAGTGGTCCAAG GCTGCCCCCATGCTGGTGGCCAGGTTTGGCCATGGATCTGCTGAACTGAAGCACTGCCTCTATGTAGTCG GTGGGCACACAGCTGCAACTGGCTGCCTCCCAGCCTCCCCCTCGGTCTCCCTAAAGCAAGTAGAACAGTA TGACCCCACAACCAACAAATGGACCATGGTAGCCCCACTCCGCGAAGGTGTCAGCAATGCTGCTGTAGTG AGTGCCAAACTTAAGCTGTTTGCTTTCGGGGGTACCAGTGTGAGCCACGACAAGCTGCCCAAGGTTCAGT GTTACGATCAATGCGAGAACAGATGGTCAGTGCCGGCCACCTGTCCCCAGCCCTGGCGTTACACAGCCGC AGCTGTGCTGGGAAACCAGATTTTTATCATGGGTGGAGATACAGAGTTCTCTGCCTGCTCCGCTTACAAA TTCAATAGTGAGACTTACCAGTGGACCAAGGTGGGAGATGTGACAGCCAAGCGCATGAGCTGCCACGCCG TGGCCTCCGGGAACAAGCTTTACGTAGTTGGAGGGTACTTCGGCATTCAGCGCTGCAAGACTTTGGACTG TTACGACCCAACTTTAGATGTGTGGAACAGCATAACCACTGTTCCCTACTCTCTGATCCCTACTGCATTC GTCAGCACCTGGAAACACCTGCCTTCCTAATGTAGAGCATCCTAAAGAAAGCACGCATGAGCTCATTCTG ATGCACGGCGAGATGAGATGTCATTTCTGCTTTGGAGAAGGCAAGTTTAATGAAGAGAAAGAAAAACAGG AGCAGTTGCTACTGAGACTCCTCGAATACCATCGGCTGCACCTTGCAAACACTCTCAAGTGGACATGAAG GAAGGGGTGGGGGGAGGGGCGGGTTTTTTTTTTTTTCAATATAAGTAGCACATGGTTTAA
	nt 1 - nt 2300 of U65079.1
Notes:	The Enc1 probe used in this study by Hernandez et al., 1997 [PMID:9096139] is described as follows: "the antisense ENC-1 riboprobe was synthesized using a digoxigenin RNA labeling kit (Boehringer Mannheim) and DNA from the clone p10.2x." Editors Note: see Fig2 therein and U65079.1 for the insert sequence of clone p10.2x. Note that U65079.1 does not include the long poly(A) tail shown in Fig2.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Age:	10.5 dpc
Theiler Stage:	TS17
Mutations:	none (wild-type)
Preparation:	wholemount

Procedures

Staining procedure:

alkaline phosphatase + unspecified

General Information

Authors:	Hernandez MC; Andres-Barquin PJ; Martinez S; Bulfone A; Rubenstein JL ; Israel MA, 1997 [PMID:9096139] , Indexed and Spatially Mapped by EMAGE.
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	non-screen
References:	[ PMID:9096139] Hernandez MC, Andres-Barquin PJ, Martinez S, Bulfone A, Rubenstein JL, Israel MA 1997 ENC-1: a novel mammalian kelch-related gene specifically expressed in the nervous system encodes an actin-binding protein. J Neurosci (17):3038-51
Links:	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source