EMAGE mouse gene expression spatial database

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EMAGE:5254

Enc1 ectodermal-neural cortex 1 ( MGI:109610)
TS19 (11.5 dpc)
in situ hybridisation

Data Images


Fig6a. Copyright: Reprinted with permission from the Society for Neuroscience from J Neurosci 17(9): 3038-51. Hernandez MC; Andres-Barquin PJ; Martinez S; Bulfone A; Rubenstein JL ; Israel MA. ENC-1: a novel mammalian kelch-related gene specifically expressed in the nervous system encodes an actin-binding protein. Copyright 1997. [PMID:9096139]	Fig6b. Copyright: Reprinted with permission from the Society for Neuroscience from J Neurosci 17(9): 3038-51. Hernandez MC; Andres-Barquin PJ; Martinez S; Bulfone A; Rubenstein JL ; Israel MA. ENC-1: a novel mammalian kelch-related gene specifically expressed in the nervous system encodes an actin-binding protein. Copyright 1997. [PMID:9096139]	Fig6c. Copyright: Reprinted with permission from the Society for Neuroscience from J Neurosci 17(9): 3038-51. Hernandez MC; Andres-Barquin PJ; Martinez S; Bulfone A; Rubenstein JL ; Israel MA. ENC-1: a novel mammalian kelch-related gene specifically expressed in the nervous system encodes an actin-binding protein. Copyright 1997. [PMID:9096139]	Fig6d. Copyright: Reprinted with permission from the Society for Neuroscience from J Neurosci 17(9): 3038-51. Hernandez MC; Andres-Barquin PJ; Martinez S; Bulfone A; Rubenstein JL ; Israel MA. ENC-1: a novel mammalian kelch-related gene specifically expressed in the nervous system encodes an actin-binding protein. Copyright 1997. [PMID:9096139]	Fig6e Copyright: Reprinted with permission from the Society for Neuroscience from J Neurosci 17(9): 3038-51. Hernandez MC; Andres-Barquin PJ; Martinez S; Bulfone A; Rubenstein JL ; Israel MA. ENC-1: a novel mammalian kelch-related gene specifically expressed in the nervous system encodes an actin-binding protein. Copyright 1997. [PMID:9096139]

Fig6f. Copyright: Reprinted with permission from the Society for Neuroscience from J Neurosci 17(9): 3038-51. Hernandez MC; Andres-Barquin PJ; Martinez S; Bulfone A; Rubenstein JL ; Israel MA. ENC-1: a novel mammalian kelch-related gene specifically expressed in the nervous system encodes an actin-binding protein. Copyright 1997. [PMID:9096139]

Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Notes:

Isolated neural tube from an E11.5 mouse embryo processed by whole-mount in situ hybridization. (a) - lateral view, (e) - dorsal view after opening of the rhombencephalic roof. (b-d), (f), sagittal sections of the same embryo. Image annotations: The arrow in (a) indicates the mammillary region (MA). In (b), the large arrow shows that the telencephalic expression of ENC-1 is localized in the mantle layer of the cortex (primordial plexiform layer (PP)), and the short arrow shows that the unique ventricular expression is localized in the anlage of the HP. The dashed line shows the location of the zona limitans (ZL). The asterisk in (d) shows the ventricular expression in the diencephalic basal plate from the rostral MB to the MA region. The bidirectional arrows show the position of interneuromeric boundaries. The large arrows show the basal plate of P3 and P4. The dotted line labels the position of the zona limitans. RH - Rhombencephalon; MB - Midbrain; CB - Cerebellar plate; I - Isthmus; IN - Infundibulum; MGE - Medial ganglionic eminence; LGE - Lateral ganglionic eminence; CxA - Cortical telencephalic anlage; P1-P6 - Prosomeres 1-6; R1-R8 - Rhombomeres 1-8; T- Telencephalon; EMT - Eminentia thalami; ET - Epithalamus; PP - Primordial plexiform layer; CB- Cerebellar plate; IF - Isthmic fosa; SC - Spinal cord; NE - Neuroepithelial zone; ML - Mantle layer.

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

5254_wholemount__moderate.wlz

5254_wholemount__weak.wlz

5254_wholemount__notDetected.wlz

5254_wholemount__strong.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:5254_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

telencephalon

strong

regional

ENC-1 expression was strong in the cortical anlage of the telencephalon and in the medial ganglionic eminence.

diencephalon

detected

regional

A transverse zone of ENC-1 expression between P1 and P2, in the region of the retroflexus tract, was recognizable.

forebrain

detected

regional

In the basal plate of the prosencephalon, the expression extended from P1 to P4, where the mammillary region (MA) was more intensely labeled.

cerebellum primordium

detected

ENC-1 was expressed in the cerebellar plate.

metencephalon alar plate

detected

ENC-1 was expressed in the cerebellar plate and in the alar plate of rhombencephalon and spinal cord.

neural tube alar columns

detected

ENC-1 was expressed in the alar plate of the spinal cord

Annotation Validation:

EMAGE Editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	Enc1 probeA
Entity Detected:	Enc1, ectodermal-neural cortex 1 ( MGI:109610)
Sequence:	sense strand is shown >Enc1 probeA CGCACGGTCACTCTCCCCTGGCTAGGCGCGCGCCGGCAGACGCTTGGCCCGGCCGGGGCGCCAGGTCCGT AGCAGCAGATCCGGAGACCGAGGTCAGCTGCCTGCCGAAACCAGCCCGGGAGCCACTACCCCGGACGCTA TCGCCTCCCTTCCCAGCGCTCCTTTCTCCCCGGCGATCGCCACCGCTTCCTGGAACAGAGACGCCCTTCG TCGGCTCCCTGCGGTGACCGAGGCAAGAACCGCAGCCGGTTGTGTGCAGAGGCCAGCCGCCACTACTGAG TTGAAACAAAATGTCAGTCAGTGTGCATGAGAACCGCAAGTCCAGGGCCAGCAGTGGCTCCATCAACATC TACCTGTTTCATAAGTCCTCCTACGCGGACAGCGTTCTCACTCACCTGAACCTTCTGCGTCAGCAGCGGC TCTTCACAGATGTCCTTCTCCATGCGGGAAACAGGACCTTCCCTTGCCACCGGGCAGTGCTGGCTGCGTG CAGCCGCTACTTCGAAGCCATGTTCAGTGGTGGCCTGAAAGAGAGCCAGGACAGTGAGGTGAACTTCGAC AATTCCATCCACCCAGAAGTCTTAGAGCTGCTTCTAGACTACGCATACTCCTCCCGGGTCATTATCAATG AAGAAAATGCTGAGTCGCTCCTGGAGGCTGGTGACATGCTGGAGTTCCAGGACATCAGAGATGCGTGTGC AGAATTTCTAGAGAAGAACCTGCATCCCACCAACTGCCTGGGTATGCTGCTGTTGTCTGATGCCCACCAG TGCACCAAGCTGTACGAACTCTCCTGGAGAATGTGTCTCAGCAACTTCCAAACCATTCGGAAGAGCGAAG ATTTCCTCCAGTTGCCCCAGGACATGGTTGTGCAGCTGCTGTCCAGTGAAGAACTGGAGACGGAAGACGA AAGGCTGGTGTATGAGTCTGCGATGAACTGGATCAGCTATGACCTGAAGAAGCGCTACTGTTACCTCCCG GAACTGTTGCAGACAGTGAGGCTGGCCCTCCTTCCTGCCATCTATCTCATGGAGAACGTGGCGATGGAAG AACTCATCACCAAGCAGAGAAAGAGTAAGGAGATCGTGGAAGAGGCCATCAGGTGCAAACTAAAAATCTT ACAGAATGACGGCGTGGTCACCAGTCTCTGTGCTCGTCCTCGGAAAACTGGCCATGCCCTGTTCCTCCTG GGAGGGCAGACTTTCATGTGTGACAAACTGTACTTGGTAGACCAGAAGGCTAAAGAAATCATTCCCAAGG CTGACATTCCCAGCCCGAGGAAAGAGTTCAGCGCATGTGCAATTGGCTGCAAAGTATATATTACTGGGGG GCGGGGATCAGAGAACGGAGTCTCAAAAGATGTCTGGGTTTACGATACCCTGCATGAGGAGTGGTCCAAG GCTGCCCCCATGCTGGTGGCCAGGTTTGGCCATGGATCTGCTGAACTGAAGCACTGCCTCTATGTAGTCG GTGGGCACACAGCTGCAACTGGCTGCCTCCCAGCCTCCCCCTCGGTCTCCCTAAAGCAAGTAGAACAGTA TGACCCCACAACCAACAAATGGACCATGGTAGCCCCACTCCGCGAAGGTGTCAGCAATGCTGCTGTAGTG AGTGCCAAACTTAAGCTGTTTGCTTTCGGGGGTACCAGTGTGAGCCACGACAAGCTGCCCAAGGTTCAGT GTTACGATCAATGCGAGAACAGATGGTCAGTGCCGGCCACCTGTCCCCAGCCCTGGCGTTACACAGCCGC AGCTGTGCTGGGAAACCAGATTTTTATCATGGGTGGAGATACAGAGTTCTCTGCCTGCTCCGCTTACAAA TTCAATAGTGAGACTTACCAGTGGACCAAGGTGGGAGATGTGACAGCCAAGCGCATGAGCTGCCACGCCG TGGCCTCCGGGAACAAGCTTTACGTAGTTGGAGGGTACTTCGGCATTCAGCGCTGCAAGACTTTGGACTG TTACGACCCAACTTTAGATGTGTGGAACAGCATAACCACTGTTCCCTACTCTCTGATCCCTACTGCATTC GTCAGCACCTGGAAACACCTGCCTTCCTAATGTAGAGCATCCTAAAGAAAGCACGCATGAGCTCATTCTG ATGCACGGCGAGATGAGATGTCATTTCTGCTTTGGAGAAGGCAAGTTTAATGAAGAGAAAGAAAAACAGG AGCAGTTGCTACTGAGACTCCTCGAATACCATCGGCTGCACCTTGCAAACACTCTCAAGTGGACATGAAG GAAGGGGTGGGGGGAGGGGCGGGTTTTTTTTTTTTTCAATATAAGTAGCACATGGTTTAA
	nt 1 - nt 2300 of U65079.1
Notes:	The Enc1 probe used in this study by Hernandez et al., 1997 [PMID:9096139] is described as follows: "the antisense ENC-1 riboprobe was synthesized using a digoxigenin RNA labeling kit (Boehringer Mannheim) and DNA from the clone p10.2x." Editors Note: see Fig2 therein and U65079.1 for the insert sequence of clone p10.2x. Note that U65079.1 does not include the long poly(A) tail shown in Fig2.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Age:	11.5 dpc
Theiler Stage:	TS19
Mutations:	none (wild-type)
Preparation:	wholemount

Procedures

Staining procedure:

alkaline phosphatase + unspecified

General Information

Authors:	Hernandez MC; Andres-Barquin PJ; Martinez S; Bulfone A; Rubenstein JL ; Israel MA, 1997 [PMID:9096139] , Indexed and Spatially Mapped by EMAGE.
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	non-screen
References:	[ PMID:9096139] Hernandez MC, Andres-Barquin PJ, Martinez S, Bulfone A, Rubenstein JL, Israel MA 1997 ENC-1: a novel mammalian kelch-related gene specifically expressed in the nervous system encodes an actin-binding protein. J Neurosci (17):3038-51
Links:	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source