Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:5255

Eng endoglin ( MGI:95392)
TS15 (9.5 dpc)
immunohistochemistry

Data Images
EMAGE:5255
Fig7Be of [doi:10.1128/MCB.23.5.1817-1831.2003] Mol Cell Biol 2003 23(5):1817-1831 [PMID:12588999] Takahashi T; Takahashi K; St John PL; Fleming PA; Tomemori T; Watanabe T; Abrahamson DR; Drake CJ; Shirasawa T; Daniel TO, "A mutant receptor tyrosine phosphatase, CD148, causes defects in vascular development." © ASM. Reproduced with permission from Molecular and Cellular Biology. Reuse of any Licensed Material requires permission from ASM. Copyright 2003.

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:5255Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
5255_wholemount__moderate.wlz
5255_wholemount__weak.wlz
5255_wholemount__notDetected.wlz
5255_wholemount__strong.wlz
(what is wlz format?)
Download all expression domains: EMAGE:5255_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
cardiovascular system
detected detected
Expression was detected in the intraembryonic vessels.
extraembryonic vascular system
detected detected
regionalExpressed in yolk sac vessels
Annotation Validation: EMAGE Editor
Detection Reagent
Type:antibody
Identifier:Eng antibodyA
Entity Detected:Eng, endoglin ( MGI:95392)
Notes:The anti-Eng (CD105/endoglin) antibody used in this study by Takahashi et al., 2003 [PMID:12588999] is described as "antiendoglin monoclonal antibody (clone MJ7/18 (Pharmingen))(1.7ug/ml)." Editors note: the additional information here was taken from the Pharmingen/BD Biosciences product sheet for clone MJ7/18 (http://www.bdbeurope.com/).
Antibody Type:monoclonal
Raised In:rat
Supplier:BD Biosciences
Catalogue Number:550546
Specimen
Organism:mouse
Age:9.5 dpc
Theiler Stage:TS15
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
Secondary Antibody:donkey anti-rat IgG
Labelled with:horse radish peroxidase
Visualisation method:DAB
General Information
Authors:Takahashi T; Takahashi K; St John PL; Fleming PA; Tomemori T; Watanabe T; Abrahamson DR; Drake CJ; Shirasawa T; Daniel TO, 2003 [PMID:12588999] , Indexed and Spatially Mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1128/MCB.23.5.1817-1831.2003] [ PMID:12588999] Takahashi T, Takahashi K, St John PL, Fleming PA, Tomemori T, Watanabe T, Abrahamson DR, Drake CJ, Shirasawa T, Daniel TO 2003 A mutant receptor tyrosine phosphatase, CD148, causes defects in vascular development. Mol Cell Biol (23):1817-31
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE