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EMAGE:5257

Enpp2 ectonucleotide pyrophosphatase/phosphodiesterase 2 ( MGI:1321390)
TS15 (9.5 dpc)
in situ hybridisation

Data Images
EMAGE:5257
Fig1F. [doi:10.1002/dvdy.21119] Copyright: This image is from Ohuchi H; Hayashibaral Y; Matsuda H; Onoi M; Mitsumori M; Tanaka M; Aoki J; Arai H; Noji S, "Diversified expression patterns of autotaxin, a gene for phospholipid-generating enzyme during mouse and chicken development." Dev Dyn 2007 Mar 15;236(4):1134-1143. Reprinted with permission of Wiley-Liss Inc. [PMID:17366625] .

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Image annotations: Red arrowhead indicates the midbrain-hindbrain boundary; h - heart; ov - otic vesicle.
Expression Pattern Description
Spatial Annotation:
EMAGE:5257Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
5257_wholemount__moderate.wlz
5257_wholemount__notDetected.wlz
5257_wholemount__strong.wlz
(what is wlz format?)
Download all expression domains: EMAGE:5257_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
future midbrain
weak weak
regionalATX expression was still detected but seemed much down-regulated in the vicinity of the MHB (compared to previous stages). ATX was weakly expressed at the posterior end of the midbrain.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:Enpp2 probeA
Entity Detected:Enpp2, ectonucleotide pyrophosphatase/phosphodiesterase 2 ( MGI:1321390)
Notes:The Enpp2 (Atx) probe used in this study by Ohuchi et al., 2007 [PMID:17366625] is indicated as that previously described by Tanaka et al., 2006 [PMID:16829511] who in turn refer to Iseki et al., 1997 [PMID:9310332] . There is however no description of an Atx probe in this last paper.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:DDY
Age:9.5 dpc
Theiler Stage:TS15
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Ohuchi H; Hayashibaral Y; Matsuda H; Onoi M; Mitsumori M; Tanaka M; Aoki J; Arai H; Noji S, 2007 [PMID:17366625] , Indexed and Spatially Mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1002/dvdy.21119] [ PMID:17366625] Ohuchi H, Hayashibara Y, Hayashibaral Y, Matsuda H, Onoi M, Mitsumori M, Tanaka M, Aoki J, Arai H, Noji S 2007 Diversified expression patterns of autotaxin, a gene for phospholipid-generating enzyme during mouse and chicken development. Dev Dyn (236):1134-43
 [ doi:10.1074/jbc.M605142200] [ PMID:16829511] Tanaka M, Okudaira S, Kishi Y, Ohkawa R, Iseki S, Ota M, Noji S, Yatomi Y, Aoki J, Arai H 2006 Autotaxin stabilizes blood vessels and is required for embryonic vasculature by producing lysophosphatidic acid. J Biol Chem (281):25822-30
 [ PMID:9310332] Iseki S, Wilkie AO, Heath JK, Ishimaru T, Eto K, Morriss-Kay GM 1997 Fgfr2 and osteopontin domains in the developing skull vault are mutually exclusive and can be altered by locally applied FGF2. Development (124):3375-84
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE