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EMAGE:526

Wnt1 wingless-related MMTV integration site 1 ( MGI:98953)
TS12 (8.5 dpc)
in situ hybridisation

Data Images
EMAGE:526 EMAGE:526
Figure 1A (middle) of Bally-Cuif et al., 1992 [PMID:1360404] Copyright: This image is from Development and is displayed with the permission of The Company of Biologists Ltd. who owns the copyright. Figure 1A (left) of Bally-Cuif et al., 1992 [PMID:1360404] Copyright: This image is from Development and is displayed with the permission of The Company of Biologists Ltd. who owns the copyright.

Expression pattern clarity: three stars
Find spatially similar expression patterns: Find spatially similar patterns
Notes:
Image 2 is a schematic representation of the section shown in Image 1. Image annotations: arrow indicates met-mesencephalic region; arrowhead indicates hindbrain.
Expression Pattern Description
Spatial Annotation:
EMAGE:526EMAGE:526Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
3D mapping3D context movie

View mapped 3D expression image EMAGE genex expression entry
Download individual expression domains:
526_voxel_strong_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:526_all_domains.zip
Find spatially similar expression patterns: EMAGE spatially similar patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
neural ectoderm
detected detected
regionalAuthors describe expression in the "neural tube" and "neural folds" in the region of the future brain "from E8.5 on " (data not shown).
future midbrain
detected detected
regionalBroad domain in the met-mesencephalic region
future hindbrain
detected detected
regionalTwo large triangular-shaped lateral patches of cells in "neural folds" on either side of the midline in the hindbrain
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:1935102
Entity Detected:Wnt1, wingless-related MMTV integration site 1 ( MGI:98953)
Notes:The Wnt1 probe used in this study by Bally-Cuif et al., 1992 [PMID:1360404] is described as "a 1879 bp HindIII-XbaI fragment of the Wnt1 cDNA (Fung et al., 1985 [PMID:3018519] )." Neither HindIII nor XbaI cut Fung's GenBank entry for Wnt1 (int-1) (M11943) although four HindIII sites (H) are indicated in Fig 3B of Fung et al., 1985.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:OF1
Age:8.5 dpc
Theiler Stage:TS12
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:4% paraformaldehyde
Embedding:paraffin
Staining procedure:alkaline phosphatase + BCIP
General Information
Authors:Bally-Cuif et al., 1992 [PMID:1360404] Indexed by GXD, Spatially mapped by EMAGE
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ PMID:1360404] Bally-Cuif L, Alvarado-Mallart RM, Darnell DK, Wassef M 1992 Relationship between Wnt-1 and En-2 expression domains during early development of normal and ectopic met-mesencephalon. Development (115):999-1009
 [ PMID:3018519] Fung YK, Shackleford GM, Brown AM, Sanders GS, Varmus HE 1985 Nucleotide sequence and expression in vitro of cDNA derived from mRNA of int-1, a provirally activated mouse mammary oncogene. Mol Cell Biol (5):3337-44
Links:MGI:1935116 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI