EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:5261

Epas1 endothelial PAS domain protein 1 ( MGI:109169)
TS17 (10.5 dpc)
in situ hybridisation

Data Images


Fig 7D. Copyright: This image is from Ema M; Taya S; Yokotani N; Sogawa K; Matsuda Y; Fujii-Kuriyama Y, Proc Natl Acad Sci U S A 1997 Apr 29;94(9):4273-8. [PMID:9113979] Copyright 1997 National Academy of Sciences, U.S.A.	Fig 7E. Copyright: This image is from Ema M; Taya S; Yokotani N; Sogawa K; Matsuda Y; Fujii-Kuriyama Y, Proc Natl Acad Sci U S A 1997 Apr 29;94(9):4273-8. [PMID:9113979] Copyright 1997 National Academy of Sciences, U.S.A.	Fig 7G. Copyright: This image is from Ema M; Taya S; Yokotani N; Sogawa K; Matsuda Y; Fujii-Kuriyama Y, Proc Natl Acad Sci U S A 1997 Apr 29;94(9):4273-8. [PMID:9113979] Copyright 1997 National Academy of Sciences, U.S.A.	Fig 7H. Copyright: This image is from Ema M; Taya S; Yokotani N; Sogawa K; Matsuda Y; Fujii-Kuriyama Y, Proc Natl Acad Sci U S A 1997 Apr 29;94(9):4273-8. [PMID:9113979] Copyright 1997 National Academy of Sciences, U.S.A.

Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Notes:

(D) Lateral view of 10.5dpc embryo hybridized with HLF cRNA probe. (E) Photograph of higher magnification (�45) of dorsal region of 10.5dpc embryo. Arrows indicate the signals in the intersomitic plexus. (G) Transverse section of 10.5-dpc embryo. Arrowheads indicate the signals in the intersomitic plexus. (H) Transverse section of the 10.5dpc embryo. An arrowhead indicates the signal of the endothelial cells of the sprouting blood vessels. Image annotations: H and G in Fig 7D refer to the levels of the sections shown in Fig7H and G respectively.

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

5261_wholemount__moderate.wlz

5261_wholemount__weak.wlz

5261_wholemount__notDetected.wlz

5261_wholemount__strong.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:5261_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

sympathetic nervous system

detected

HLF became prominent in the sympathetic ganglia

cardiovascular system

strong

regional

HLF became prominent in the sprouting vessels of the head mesenchyme. High expression in vascular endothelial cells, including the dorsal aorta and intersomitic plexus

dorsal aorta

strong

regional

High expression in vascular endothelial cells, including the dorsal aorta and intersomitic plexus

Annotation Validation:

EMAGE Editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	Epas1 probeA
Entity Detected:	Epas1, endothelial PAS domain protein 1 ( MGI:109169)
Sequence:	sense strand is shown >Epas1 probeA GCATGCCCCTGCCTGCCCCGCCGTCTTGACCTGCCAGCTTCACTTCCATCTGTGTTGCTATTAGGTATCT CTAACACCAGCACACTTCTTACGAGATGTACTCAACCTGGCCTACTGGCCAGGTCACCAAGCAGTGGCCT TTATCTGAAATGCTCACTTTATTATCCATGTTTTAAAAAAACATAGTTGTTGTACCTGCTATGTTTTACC GTTGATGAAAGTGTTCTGAAATTTAATAAGATTTTCCCCCTCCCTCCCTCCCTTGAATTACTTCTAATTT ATATTCCCCAAAGGTTTTTCTCTCTCTCATTCATATCCATACTAACAAGCATGGTGGCTGGTGCCTCTCC CTAGGAAAGCTTTGGCGTCATTCAACTCAAGTGTTCTTGTTCTTGTTGCCAAAGAGAAAAGGATTTTCCT CCACTGTGGATTCTCCCTCTCCCCCCACCCCCACATACACACAC
	nt 3025 - nt 3488 of D89787.1
Notes:	The Epas1 (mHLF) probe used in this study by Ema et al., 1997 [PMID:9113979] is described as follows: "mHLF probe (nucleotides 3025-3488)." Editors Note: the mRNA sequence for mHLF was submitted by Ema, M. as D89787.1.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Age:	10.5 dpc
Theiler Stage:	TS17
Mutations:	none (wild-type)
Preparation:	wholemount

Procedures

General Information

Authors:	Ema M; Taya S; Yokotani N; Sogawa K; Matsuda Y; Fujii-Kuriyama Y, 1997 [PMID:9113979] , Indexed and Spatially Mapped by EMAGE.
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	non-screen
References:	[ doi:10.1073/pnas.94.9.4273] [ PMID:9113979] Ema M, Taya S, Yokotani N, Sogawa K, Matsuda Y, Fujii-Kuriyama Y 1997 A novel bHLH-PAS factor with close sequence similarity to hypoxia-inducible factor 1alpha regulates the VEGF expression and is potentially involved in lung and vascular development. Proc Natl Acad Sci U S A (94):4273-8
Links:	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source