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EMAGE:5264

Epha1 Eph receptor A1 ( MGI:107381)
TS19 (11.5 dpc)
in situ hybridisation

Data Images
EMAGE:5264
Fig4g. Copyright: Reprinted with permission from Elsevier from [doi:10.1016/j.modgep.2005.12.007] Gene Expr Patterns 6(7): 719-23, Duffy SL; Steiner KA; Tam PP; Boyd AW, Expression analysis of the Epha1 receptor tyrosine kinase and its high-affinity ligands Efna1 and Efna3 during early mouse development. Copyright 2006. [PMID:16466970]

Expression pattern clarity: two stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:5264Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
5264_wholemount__possible.wlz
5264_wholemount__notDetected.wlz
5264_wholemount__strong.wlz
(what is wlz format?)
Download all expression domains: EMAGE:5264_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
tail unsegmented mesenchyme
detected detected
Epha1 expression is restricted to the tail bud mesenchyme and the posterior presomitic mesoderm.
tail mesenchyme
detected detected
Epha1 expression is restricted to the tail bud mesenchyme and the posterior presomitic mesoderm
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:Epha1 probeA
Entity Detected:Epha1, Eph receptor A1 ( MGI:107381)
Notes:The Epha1 probe used in this study by Duffy et al., 2006 [PMID:16466970] is described as follows: "The Epha1 probe corresponded to bases 2085-3106 of the coding sequence and was generated from a PCR-amplified cDNA fragment (GenBank Accession No. NM_023580, nt 2226-3247)." Editors Note: In 2006 when this paper was published, 2 versions of NM_02358 existed: NM_02358.1 (2934 bp; CDS 1..2934) and NM_02358.2 (3275 bp; CDS 60..2993). It is assumed that the authors are referring to NM_02358.2 because the the 3' end co-ordinate given (nt3247) is less than the total length of NM_02358.2 (3275nt). Additionally, NM_02358.2 replaced NM_02358.1 a number of years prior to this publication (i.e. in 2003). However, there is still a discrepancy between the two descriptions given: "bases 2085-3106 of the coding sequence" i.e. nt 2145-3166 of NM_02358.2; and "NM_023580, nt 2226-3247" i.e. nt2226-3247 of NM_02358.2. The exact sequence of the probe is unclear.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:ARC/S
Age:11.5 dpc
Theiler Stage:TS19
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:0.2% glutaraldehyde/4% PFA
Staining procedure:alkaline phosphatase + NBT/BCIP or BM purple
General Information
Authors:Duffy SL; Steiner KA; Tam PP; Boyd AW, 2006 [PMID:16466970] , Indexed and Spatially Mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1016/j.modgep.2005.12.007] [ PMID:16466970] Duffy SL, Steiner KA, Tam PP, Boyd AW 2006 Expression analysis of the Epha1 receptor tyrosine kinase and its high-affinity ligands Efna1 and Efna3 during early mouse development. Gene Expr Patterns (6):719-23
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE