Type: | in situ hybridisation probe |
Identifier: | Epha1 probeA |
Entity Detected: | Epha1, Eph receptor A1 ( MGI:107381) |
Notes: | The Epha1 probe used in this study by Duffy et al., 2006 [PMID:16466970] is described as follows: "The Epha1 probe corresponded to bases 2085-3106 of the coding sequence and was generated from a PCR-amplified cDNA fragment (GenBank Accession No. NM_023580, nt 2226-3247)."
Editors Note: In 2006 when this paper was published, 2 versions of NM_02358 existed: NM_02358.1 (2934 bp; CDS 1..2934) and NM_02358.2 (3275 bp; CDS 60..2993). It is assumed that the authors are referring to NM_02358.2 because the the 3' end co-ordinate given (nt3247) is less than the total length of NM_02358.2 (3275nt). Additionally, NM_02358.2 replaced NM_02358.1 a number of years prior to this publication (i.e. in 2003).
However, there is still a discrepancy between the two descriptions given: "bases 2085-3106 of the coding sequence" i.e. nt 2145-3166 of NM_02358.2; and "NM_023580, nt 2226-3247" i.e. nt2226-3247 of NM_02358.2. The exact sequence of the probe is unclear. |
Chemistry: | RNA |
Strand: | antisense |
Label: | digoxigenin |