EMAGE mouse gene expression spatial database

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EMAGE:5268

Epha3 Eph receptor A3 ( MGI:99612)
TS17 (10.5 dpc)
in situ hybridisation

Data Images


Fig7G. Copyright: Reprinted with permission from Cell Press from [doi:10.1016/0092-8674(94)90408-1] Cell 79(1): 157-168, Cheng HJ; Flanagan JG, Identification and cloning of ELF-1, a developmentally expressed ligand for the Mek4 and Sek receptor tyrosine kinases. Copyright 1994 [PMID:7522971]

Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Notes:

Image annotations: midbrain (large arrow), hindbrain (small arrow), first and second branchial arches (arrowheads), somites (open triangle), and limb buds (star).

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

5268_wholemount__moderate.wlz

5268_wholemount__possible.wlz

5268_wholemount__notDetected.wlz

5268_wholemount__strong.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:5268_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

trunk somite

detected

regional

Mek4 is restricted to the dorsal part, the ventral part, or both.

branchial arch

detected

regional

Expression is in a localized subregion.

limb

detected

Mek4 is expressed less prominently in the limb bud, in a posterior region near the junction of the limb buds with the flank, and in the lateral plate between the limb buds.

Annotation Validation:

EMAGE Editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	Epha3 probeA
Entity Detected:	Epha3, Eph receptor A3 ( MGI:99612)
Sequence:	sense strand is shown >Epha3 probeA GCATTCTGAAACTCAACACTGAGATTAGAGAAGTGGGACCAGTCAACAAAAAGGGGTTTTATTTGGCCTT TCAAGATGTTGGTGCTTGTGTTGCCTTGGTGTCTGTGAGAGTGTACTTCAAAAAGTGCCCGTTTACAGTG AAGAATCTGGCTATGTTTCCAGACACAGTGCCCATGGACTCCCAGTCTTTGGTGGAGGTTAGGGGCTCTT GTGTCAATAATTCCAAAGAGGAGGACCCTCCCAGGATGTACTGCAGCACAGAAGGGGAATGGCTGGTCCC CATTGGCAAATGCACTTGCAATGCTGGGTATGAAGAACGAGGTTTCATATGCCAAGCTTGTCGACCAGGC TTCTACAAGGCTTCCGATGGTGCTGCTAAGTGTGCTAAGTGCCCACCACACAGCTCAACACAAGAAGATG GTTCAATGAACTGCAGGTGTGAGAATAATTATTTCCGGGCAGAAAAAGACCCTCCATCCATGGCTTGTGC CCGACCTCCATCCGCACCAAGAAATGTTATCTCAAACATAAACGAGACCTCGGTTATCCTGGACTGGAGT TGGCCCCTGGACACAGGAGGCCGGAAGGATATTACCTTCAACATCATATGTAAAAAGTGTGGGTGGAATG TCAGGCAGTGTGAGCCGTGCAGCCCCAACGTCCGCTTCCTTCCTCGGCAGCTTGGACTCACCAACACCAC GGTGACAGTGACAGACCTCCTAGCTCACACCAACTACACCTTTGAGATCGATGCTGTTAATGGGGTGTCA GAGCTGAGCTCGCCACCGAGACAGTATGCCGCAGTCAGCATCACAACTAATCAGGCTGCTCCATCACCAG TTATGACAATTAAGAAAGATCGGACTTCCAGAAACAGTATCTCTTTATCTTGGCAAGAACCGGAACATCC AAATGGAATTATCCTGGACTATGAGGTCAAATACTATCAAAAGCAGGAGCAAGAGACGAGTTATACCATT TTGAGAGCAAGAGGCACAAATGTTACTATCAGTAGCCTCAAGCCAGATACTACTTATGTCTTCCAAATCC GAGCTCGGACAGCAGCGGGATATGGAACCAACAGCCGCAAATTTGAATTTGAAACTAGCCCAGACTCTTT CTCCATCTCCGGTGAAAACAGTCAT
	nt 564 - nt 1708 of M68513.1
Notes:	The Epha3 (Mek4) probe used in this study by Cheng & Flanagan, 1994 [PMID:7522971] is described as "a 1.1 kb fragment from a Bsml site at nucleotide 564 to the transmembrane sequence at 1708." Editors Note: Cheng & Flanagan, 1994 do not refer the start and stop co-ordinates of the probe sequence (i.e. nt564 - nt1708) to any particular published sequence, however, a BsmI site is found at the same position in the mouse Mek4 cDNA sequence M68513.1, which was published in 1993. It is therefore assumed that Cheng and Flanagan are referring to M68513.1.
Chemistry:	RNA
Strand:	antisense

Specimen

Organism:	mouse
Age:	10.5 dpc
Theiler Stage:	TS17
Mutations:	none (wild-type)
Preparation:	wholemount

Procedures

General Information

Authors:	Cheng HJ; Flanagan JG, 1994 [PMID:7522971] , Indexed and Spatially Mapped by EMAGE.
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	non-screen
References:	[ doi:10.1016/0092-8674(94)90408-1] [ PMID:7522971] Cheng HJ, Flanagan JG 1994 Identification and cloning of ELF-1, a developmentally expressed ligand for the Mek4 and Sek receptor tyrosine kinases. Cell (79):157-68
Links:	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source