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EMAGE:5269

Epha4 Eph receptor A4 ( MGI:98277)
TS18 (10.5 dpc)
in situ hybridisation

Data Images
EMAGE:5269 EMAGE:5269
Fig1E_upper. [doi:10.1242/dev.02422] Copyright: This image is from Nakajima Y; Morimoto M; Takahashi Y; Koseki H; Saga Y, Development 2006;133(13):2517-25 and is displayed with the permission of The Company of Biologist Limited who owns the Copyright. [PMID:16728472] Fig1E_lower. [doi:10.1242/dev.02422] Copyright: This image is from Nakajima Y; Morimoto M; Takahashi Y; Koseki H; Saga Y, Development 2006;133(13):2517-25 and is displayed with the permission of The Company of Biologist Limited who owns the Copyright. [PMID:16728472]

Expression pattern clarity: two stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
In situ signals in the anterior PSM are indicated by arrows in the lower panel image, which shows a magnified image.
Expression Pattern Description
Spatial Annotation:
EMAGE:5269Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
5269_wholemount__moderate.wlz
5269_wholemount__weak.wlz
5269_wholemount__notDetected.wlz
5269_wholemount__strong.wlz
5269_wholemount__possible.wlz
(what is wlz format?)
Download all expression domains: EMAGE:5269_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
limb
detected detected
branchial arch
detected detected
hindbrain
detected detected
Expressed in rhombomeres.
tail unsegmented mesenchyme
detected detected
regionalIn situ signals are detected in the anterior PSM (pre-somitic mesoderm)
somite 45
detected detected
regionalExpression was detected in "the rostral compartment of the S1 somites". Editors note: "S1 somites" is assumed to mean the most newly formed somite pair i.e. the last somite pair if numbered from the anterior as is done in the EMAP anatomy ontology.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:Epha4 probeA
Entity Detected:Epha4, Eph receptor A4 ( MGI:98277)
Notes:The Epha4 probe used in this study by Nakajima et al., 2006 [PMID:16728472] is of unreported sequence.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Age:10.5 dpc
Theiler Stage:TS18
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Staining procedure:alkaline phosphatase + BMpurple
General Information
Authors:Nakajima Y; Morimoto M; Takahashi Y; Koseki H; Saga Y, 2006 [PMID:16728472] , Indexed and Spatially Mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1242/dev.02422] [ PMID:16728472] Nakajima Y, Morimoto M, Takahashi Y, Koseki H, Saga Y 2006 Identification of Epha4 enhancer required for segmental expression and the regulation by Mesp2. Development (133):2517-25
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE