EMAGE mouse gene expression spatial database

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EMAGE:5271

Ephb2 Eph receptor B2 ( MGI:99611)
TS14 (10 Somite no.)
in situ hybridisation

Data Images


Fig5B Copyright: Reprinted with permission from Elsevier from [doi:10.1016/0925-4773(94)90091-4] Mech Dev 47(1): 3-17, Becker N; Seitanidou T; Murphy P; Mattei MG; Topilko P; Nieto MA; Wilkinson DG; Charnay P; Gilardi-Hebenstreit P, Several receptor tyrosine kinase genes of the Eph family are segmentally expressed in the developing hindbrain. Copyright 1994. [PMID:7947319]

Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Notes:

Image annotations: fg, foregut pocket; m, mesoderm; mb, midbrain; opv, optic vesicle.

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

5271_wholemount__moderate.wlz

5271_wholemount__weak.wlz

5271_wholemount__notDetected.wlz

5271_wholemount__strong.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:5271_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

future midbrain

detected

regional

expression is detected in the ventral midbrain.

prosencephalon

detected

regional

expression is detected in the ventroposterior forebrain.

optic vesicle

detected

regional

expression is detected in the posterior part of the optic vesicle.

rhombomere 05

detected

A low level of Sek-3 expression is evident in r5.

rhombomere 02

detected

Sek-3 is expressed in r2, r3, r5.

rhombomere 03

detected

Sek-3 is expressed in r2, r3, r5.

foregut

weak

regional

Low level expression in the lining of the posterior half of the foregut pocket

trunk mesenchyme

weak

regional

Low level expression is observed in the mesoderm immediately posterior to the heart.

Annotation Validation:

Editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	Ephb2 probeA
Entity Detected:	Ephb2, Eph receptor B2 ( MGI:99611)
Sequence:	sense strand is shown >Ephb2 probeA GTGCGGGCACGCACCGTTGCAGGCTATGGGCGCTACAGTGGCAAGATGTACTTCCAAACCATGACAGAAG CCGAGTACCAGACCAGCATCAAGGAAAAGCTACCCCTCATCGTTGGCTCCTCCGCCGCCGGCTTAGTCTT CCTCATCGCTGTGGTCGTCATTGCCATCGTATGTAACAGAAGACGGGGGTTTGAGCGTGCCGACTCAGAG TACACGGACAAGCTACAGCACTACACCAGCGGACACATGACCCCAGGCATGAAGATCTATATAGATCCTT TCACCTATGAAGATCCTAATGAGGCAGTGCGGGAGTTTGCCAAGGAAATTGACATCTCCTGTGTCAAGAT TGAGCAGGTGATTGGAGCAGGGGAATTTGGTGAGGTCTGCAGTGGCCATTTGAAGCTGCCAGGCAAGAGA GAGATCTTTGTAGCCATCAAGACCCTCAAGTCAGGATACACGGAGAAACAGCGCCGGGACTTCCTGAGTG AGGCATCCATCATGGGCCAGTTCGACCACCCCAATGTCATCCATCTGGAAGGGGTTGTCACCAAGAGCAC ACCTGTCATGATCATCACTGAATTCATGGAGAATGGATCTCTGGACTCCTTCCTCCGGCAAAATGATGGG CAGTTCACAGTCATCCAACTGGTGGGCATGCTGAGGGGCATTGCAGCCGGCATGAAGTACCTGGCGGACA TGAACTACGTGCACCGTGACCTTGCTGCTCGAAACATCCTCGTCAACAGTAACCTGGTGTGTAAGGTGTC TGACTTTGGGCTCTCACGCTTCCTGGAGGATGACACGTCTGACCCCACCTATACCAGCGCTCTGGGTGGG AAGATCCCCATCCGTTGGACGGCACCGGAAGCCATCCAGTACCGGAAATTCACCTCGGCCAGTGATGTGT GGAGCTATGGCATCGTCATGTGGGAGGTGATGTCCTACGGGGAACGACCCTACTGGGACATGACCAATCA AGACGTAATCAACGCCATTGAACAGGACTACAGACTACCTCCGCCCATGGACTGCCCTAGCGCCCTGCAC CAGCTCATGCTGGACTGCTGGCAGAAGGACCGCAACCACCGGCCCAAGTTCGGCCAGATTGTCAACACGC TGGACAAGATGATCCGAAACCCCAACAGCCTCAAAGCCATGGCACCCCTGTCCTCTGGCATCAACCTGCC ACTGCTGGACCGCACGATACCGGACTACACCAGCTTTAACACAGTGGATGAGTGGCTAGAGGCCATCAAG ATGGGCCAGTACAAGGAGAGCTTTGCCAACGCCGGCTTCACCTCTTTCGACGTTGTATCTCAGATGATGA TGGAGGACATTCTCCGCGTTGGGGTCACTCTAGCTGGCCACCAGAAAAAAATCCTGAACAGTATCCAGGT GATGCGGGCCCAGATGAACCAGATCCAGTCTGTAGAGGTTTGACATTCGCCTGCCTCGGTTCTCCTCTTC CTCCACGCCGCCCCCTGAGCCCCCCTACGTCGGTCCCCCTGCTGCTCTGTCCACTGCAAGGTCAGCCACC TGCCAGGAGGCCACAGACAACAGGAAGAACCAAGCAGCACTCCAGCCAAGCCGTCACCAAGAGCCATGCA AGTCAAGGAGGAAGGGATGGTGGGG
Notes:	The Ephb2 (Sek-3) probe used in this study by Becker et al., 1994 [PMID:7947319] is described as "2.6 kb long, and corresponds to the entire cloned cDNA." Editors Note: See Fig 1A therein for a cartoon representation of the 2.6kb Sek-3 cDNA clone. As indicated, around 1kb of the 3'-most sequence was not determined. X76011.1 is the GenBank sequence submission from Becker to accompany this paper and corresponds to the rest of the sequence (1.6kb) of this clone.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	OF1
Age:	10 Somite no.
Theiler Stage:	TS14
Mutations:	none (wild-type)
Preparation:	wholemount

Procedures

Fixation:	4% paraformaldehyde
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Becker N; Seitanidou T; Murphy P; Mattei MG; Topilko P; Nieto MA; Wilkinson DG; Charnay P; Gilardi-Hebenstreit P, 1994 [PMID:7947319] , Indexed and Spatially Mapped by EMAGE.
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	non-screen
References:	[ doi:10.1016/0925-4773(94)90091-4] [ PMID:7947319] Becker N, Seitanidou T, Murphy P, Mattei MG, Topilko P, Nieto MA, Wilkinson DG, Charnay P, Gilardi-Hebenstreit P 1994 Several receptor tyrosine kinase genes of the Eph family are segmentally expressed in the developing hindbrain. Mech Dev (1994):3-17
Links:	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source