EMAGE mouse gene expression spatial database

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EMAGE:5272

Ephb2 Eph receptor B2 ( MGI:99611)
TS15 (20-25 Somite no.)
in situ hybridisation

Data Images


Fig5H Copyright: Reprinted with permission from Elsevier from [doi:10.1016/0925-4773(94)90091-4] Mech Dev 47(1): 3-17, Becker N; Seitanidou T; Murphy P; Mattei MG; Topilko P; Nieto MA; Wilkinson DG; Charnay P; Gilardi-Hebenstreit P, Several receptor tyrosine kinase genes of the Eph family are segmentally expressed in the developing hindbrain. Copyright 1994. [PMID:7947319]

Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Notes:

Image annotations: fg - foregut.

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

5272_wholemount__moderate.wlz

5272_wholemount__weak.wlz

5272_wholemount__notDetected.wlz

5272_wholemount__strong.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:5272_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

future midbrain

strong

regional

Sek-3 expression is detected in the ventral midbrain.

diencephalon

strong

High level Sek-3 expression persists in the ventral diencephalon.

optic vesicle

strong

regional

Sek-3 expression is detected in the posterior part of the optic vesicle.

telencephalon

strong

regional

High level Sek-3 expression persists in the posterior ventral part of the telencephalon.

dorsal root ganglion

weak

The embryo shown was subjected to a longer incubation with the alkaline phosphatase substrate in order to reveal the low levels of Sek-3 RNA in dorsal root ganglia

Annotation Validation:

EMAGE Editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	Ephb2 probeA
Entity Detected:	Ephb2, Eph receptor B2 ( MGI:99611)
Sequence:	sense strand is shown >Ephb2 probeA GTGCGGGCACGCACCGTTGCAGGCTATGGGCGCTACAGTGGCAAGATGTACTTCCAAACCATGACAGAAG CCGAGTACCAGACCAGCATCAAGGAAAAGCTACCCCTCATCGTTGGCTCCTCCGCCGCCGGCTTAGTCTT CCTCATCGCTGTGGTCGTCATTGCCATCGTATGTAACAGAAGACGGGGGTTTGAGCGTGCCGACTCAGAG TACACGGACAAGCTACAGCACTACACCAGCGGACACATGACCCCAGGCATGAAGATCTATATAGATCCTT TCACCTATGAAGATCCTAATGAGGCAGTGCGGGAGTTTGCCAAGGAAATTGACATCTCCTGTGTCAAGAT TGAGCAGGTGATTGGAGCAGGGGAATTTGGTGAGGTCTGCAGTGGCCATTTGAAGCTGCCAGGCAAGAGA GAGATCTTTGTAGCCATCAAGACCCTCAAGTCAGGATACACGGAGAAACAGCGCCGGGACTTCCTGAGTG AGGCATCCATCATGGGCCAGTTCGACCACCCCAATGTCATCCATCTGGAAGGGGTTGTCACCAAGAGCAC ACCTGTCATGATCATCACTGAATTCATGGAGAATGGATCTCTGGACTCCTTCCTCCGGCAAAATGATGGG CAGTTCACAGTCATCCAACTGGTGGGCATGCTGAGGGGCATTGCAGCCGGCATGAAGTACCTGGCGGACA TGAACTACGTGCACCGTGACCTTGCTGCTCGAAACATCCTCGTCAACAGTAACCTGGTGTGTAAGGTGTC TGACTTTGGGCTCTCACGCTTCCTGGAGGATGACACGTCTGACCCCACCTATACCAGCGCTCTGGGTGGG AAGATCCCCATCCGTTGGACGGCACCGGAAGCCATCCAGTACCGGAAATTCACCTCGGCCAGTGATGTGT GGAGCTATGGCATCGTCATGTGGGAGGTGATGTCCTACGGGGAACGACCCTACTGGGACATGACCAATCA AGACGTAATCAACGCCATTGAACAGGACTACAGACTACCTCCGCCCATGGACTGCCCTAGCGCCCTGCAC CAGCTCATGCTGGACTGCTGGCAGAAGGACCGCAACCACCGGCCCAAGTTCGGCCAGATTGTCAACACGC TGGACAAGATGATCCGAAACCCCAACAGCCTCAAAGCCATGGCACCCCTGTCCTCTGGCATCAACCTGCC ACTGCTGGACCGCACGATACCGGACTACACCAGCTTTAACACAGTGGATGAGTGGCTAGAGGCCATCAAG ATGGGCCAGTACAAGGAGAGCTTTGCCAACGCCGGCTTCACCTCTTTCGACGTTGTATCTCAGATGATGA TGGAGGACATTCTCCGCGTTGGGGTCACTCTAGCTGGCCACCAGAAAAAAATCCTGAACAGTATCCAGGT GATGCGGGCCCAGATGAACCAGATCCAGTCTGTAGAGGTTTGACATTCGCCTGCCTCGGTTCTCCTCTTC CTCCACGCCGCCCCCTGAGCCCCCCTACGTCGGTCCCCCTGCTGCTCTGTCCACTGCAAGGTCAGCCACC TGCCAGGAGGCCACAGACAACAGGAAGAACCAAGCAGCACTCCAGCCAAGCCGTCACCAAGAGCCATGCA AGTCAAGGAGGAAGGGATGGTGGGG
Notes:	The Ephb2 (Sek-3) probe used in this study by Becker et al., 1994 [PMID:7947319] is described as "2.6 kb long, and corresponds to the entire cloned cDNA." Editors Note: See Fig 1A therein for a cartoon representation of the 2.6kb Sek-3 cDNA clone. As indicated, around 1kb of the 3'-most sequence was not determined. X76011.1 is the GenBank sequence submission from Becker to accompany this paper and corresponds to the rest of the sequence (1.6kb) of this clone.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	OF1
Age:	20-25 Somite no.
Theiler Stage:	TS15
Mutations:	none (wild-type)
Preparation:	wholemount

Procedures

Fixation:	4% paraformaldehyde
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Becker N; Seitanidou T; Murphy P; Mattei MG; Topilko P; Nieto MA; Wilkinson DG; Charnay P; Gilardi-Hebenstreit P, 1994 [PMID:7947319] , Indexed and Spatially Mapped by EMAGE.
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	non-screen
References:	[ doi:10.1016/0925-4773(94)90091-4] [ PMID:7947319] Becker N, Seitanidou T, Murphy P, Mattei MG, Topilko P, Nieto MA, Wilkinson DG, Charnay P, Gilardi-Hebenstreit P 1994 Several receptor tyrosine kinase genes of the Eph family are segmentally expressed in the developing hindbrain. Mech Dev (1994):3-17
Links:	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source