EMAGE mouse gene expression spatial database

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EMAGE:5274

Ephb3 Eph receptor B3 ( MGI:104770)
TS15 (25 Somite no.)
in situ hybridisation

Data Images


Fig5G Copyright: Reprinted with permission from Elsevier from [doi:10.1016/0925-4773(94)90091-4] Mech Dev 47(1): 3-17, Becker N; Seitanidou T; Murphy P; Mattei MG; Topilko P; Nieto MA; Wilkinson DG; Charnay P; Gilardi-Hebenstreit P, Several receptor tyrosine kinase genes of the Eph family are segmentally expressed in the developing hindbrain. Copyright 1994. [PMID:7947319]

Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Notes:

Image annotations: b1 - branchial arch 1; fl - forelimb bud; fm - facial mesenchyme.

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

5274_wholemount__moderate.wlz

5274_wholemount__weak.wlz

5274_wholemount__notDetected.wlz

5274_wholemount__strong.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:5274_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

head mesenchyme

detected

regional

Expression is detected in the facial mesenchyme.

1st branchial arch

detected

graded

staining is most intense in the anterior half of the first branchial arch.

2nd branchial arch

detected

trunk mesenchyme

detected

regional

Expression is detected in the lateral mesoderm at the level of the forelimb bud.

forelimb bud

possible

Expression is detected in the lateral mesoderm at the level of the forelimb bud.

Annotation Validation:

EMAGE Editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	Ephb3 probeB
Entity Detected:	Ephb3, Eph receptor B3 ( MGI:104770)
Sequence:	sense strand is shown >Ephb3 probeB CAGCTCAATGACGGACAGTTCACGGTCATCCAGCTTGTGGGCATGTTACGAGGCATTGCTGCTGGCATGA AGTACTTGTCTGAGATGAACTACGTGCACCGTGACCTTGCTGCCCGAAACATCCTTGTCAACAGCAACTT GGTCTGCAAAGTCTCTGACTTTGGCCTCTCCCGCTTCCTGGAGGATGACCCATCAGACCCCACCTACACC AGCTCCCTGGGTGGGAAGATCCCTATCCGCTGGACCGCCCCGGAGGCCATAGCCTATCGGAAGTTTACGT CTGCCAGCGATGTCTGGAGCTATGGGATTGTCATGTGGGAGGTCATGAGCTACGGAGAGCGACCCTACTG GGATATGAGCAACCAGGATGTCATTAATGCTGTAGAGCAAGACTATCGGTTACCACCCCCCATGGACTGC CCCACCGCGCTGCACCAGCTCATGCTGGACTGTTGGGTGCGGGACCGGAACCTCAGGCCCAAGTTCTCTC AAATCGTCAACACACTAGACAAACTTATCCGCAATGCTGCCAGCCTCAAGGTCACCGCCAGTGCCCCATC TGGCATGTCCCAGCCCCTCCTGGACCGCACAGTCCCAGATTATACGACCTTCACGACAGTGGGTGACTGG CTAGATGCCATCAAGATGGGGAGGTATAAAGAGAGCTTTGTCGGTGCTGGGTTTGCATCCTTTGACCTGG TGGCCCAGATGACTGCAGAAGATCTGCTAAGGATTGGGGTCACTTTGGCTGGGCACCAGAAGAAGATCCT CTGCAGTATCCAGGACATGCGGCTGCAGATGAACCAGACCCTGCCTGTGCAGGTCTGACACTCAGCTCCA ACGAGGGGGGGGTGCCCCCCCAGGACTGCACAAGGATTCTGACCAGCCAGCTGGACTTTTGGATGCCTGG CCTCTGGCTGTGGCCCAGAAGATGGAAGTTTGGGGGAGGACCCTAGCTGTGACTTCTCCAGGCCTGTGCT CCCTCCCAGGAAGTGTGCCCCAAACCTCTTCATATTGAAGATGGATTAGAAGAGGGGGTGATGTCCCCTC CCCAGATGCCTTGGGGCCCGGTACCCAATTCGCCCTATAGTGAGTCGTATTACAATTCACTGGCCGTCGT TTTACAAC
Notes:	The Ephb3 (Sek-4) probe used in this study by Becker et al., 1994 [PMID:7947319] is described as "1.6 kb long, and corresponds to the entire cloned cDNA." Editors Note: See Fig 1A therein for a cartoon representation of the 1.6kb Sek-4 cDNA clone. As indicated, around 0.5kb of the 3'-most sequence was not determined. X76012.1 is the GenBank sequence submission from Becker to accompany this paper and corresponds to the rest of the sequence (1.1kb) of this clone.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	OF1
Age:	25 Somite no.
Theiler Stage:	TS15
Mutations:	none (wild-type)
Preparation:	wholemount

Procedures

Fixation:	4% paraformaldehyde
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Becker N; Seitanidou T; Murphy P; Mattei MG; Topilko P; Nieto MA; Wilkinson DG; Charnay P; Gilardi-Hebenstreit P, 1994 [PMID:7947319] , Indexed and Spatially Mapped by EMAGE.
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	non-screen
References:	[ doi:10.1016/0925-4773(94)90091-4] [ PMID:7947319] Becker N, Seitanidou T, Murphy P, Mattei MG, Topilko P, Nieto MA, Wilkinson DG, Charnay P, Gilardi-Hebenstreit P 1994 Several receptor tyrosine kinase genes of the Eph family are segmentally expressed in the developing hindbrain. Mech Dev (1994):3-17
Links:	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source