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EMAGE:5278

Wnt9a wingless-type MMTV integration site 9A ( MGI:2446084)
TS19 (11.5 dpc)
in situ hybridisation

Data Images
EMAGE:5278
Fig1A_Wnt14_11.5dpc. [doi:10.1101/gad.1230704] Guo X; Day TF; Jiang X; Garrett-Beal L; Topol L; Yang Y, "Wnt/beta-catenin signaling is sufficient and necessary for synovial joint formation." Genes Dev 2004 Oct 1;18(19):2404-17. [PMID:15371327]

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:5278Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
5278_wholemount__moderate.wlz
5278_wholemount__weak.wlz
5278_wholemount__notDetected.wlz
5278_wholemount__strong.wlz
(what is wlz format?)
Download all expression domains: EMAGE:5278_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
future arm mesenchyme
detected detected
regionalExpression is in the future shoulder and elbow joints, as well as in mesenchymal cells surrounding forming cartilage.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:3487150
Entity Detected:Wnt9a, wingless-type MMTV integration site 9A ( MGI:2446084)
Notes:The Wnt9a (Wnt14) probe template used in this study by Guo et al., 2004 [PMID:15371327] was a "full-length cDNA of Wnt14, generated by PCR and subcloned into the pBluescript vector. The cDNA was verified by sequencing and used to generate probes for in situ hybridization". Editors note: the primer sequences or actual end points of this "full-length cDNA" are not described.
Chemistry:RNA
Strand:antisense
Specimen
Organism:mouse
Age:11.5 dpc
Theiler Stage:TS19
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
General Information
Authors:Guo X; Day TF; Jiang X; Garrett-Beal L; Topol L; Yang Y, 2004 [PMID:15371327] . Indexed by GXD, Spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1101/gad.1230704] [ PMID:15371327] Guo X, Day TF, Jiang X, Garrett-Beal L, Topol L, Yang Y 2004 Wnt/beta-catenin signaling is sufficient and necessary for synovial joint formation. Genes Dev (18):2404-17
Links:MGI:3510316 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI