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EMAGE:5283

Ywhah tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, eta polypeptide ( MGI:109194)
TS16 (10.5 dpc)
in situ hybridisation

Data Images
EMAGE:5283
Figure 4A. Tang SJ; Suen TC; McInnes RR; Buchwald M, "Association of the TLX-2 homeodomain and 14-3-3eta signaling proteins." J Biol Chem 1998, 273: 25356-63. [PMID:9738002]

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:5283Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
5283_wholemount__moderate.wlz
5283_wholemount__weak.wlz
5283_wholemount__possible.wlz
5283_wholemount__notDetected.wlz
5283_wholemount__strong.wlz
(what is wlz format?)
Download all expression domains: EMAGE:5283_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
cranial ganglion
detected detected
dorsal root ganglion
detected detected
branchial arch
detected detected
limb
detected detected
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:Ywhah probeA
Entity Detected:Ywhah, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, eta polypeptide ( MGI:109194)
Notes:The Ywhah (14-3-3eta) probe used in this study by Tang et al., 1998 [PMID:9738002] was generated from "pEXlox(+)-14-3-3eta", which was "linearized with BglII and transcribed with Sp6 polymerase". pEXlox(+)-14-3-3eta is "a mouse 14-3-3eta cDNA plasmid derived from lambda EXlox-14-3-3eta (phage) after Cre-mediated excision". Editors Note: In the results section, the authors report the cloning of a 1668bp 14-3-3eta clone from "a cDNA expression library derived from mouse embryos 10.0 days postcoitus (Novagen)" - see Fig 1 therein and AF077002.1 for the insert sequence of this clone. It is not explicitly stated, but this 1668bp clone could be pEXlox(+)-14-3-3eta as EXlox vectors are manufactured by Novagen.
Chemistry:RNA
Strand:antisense
Specimen
Organism:mouse
Age:10.5 dpc
Theiler Stage:TS16
Mutations:none (wild-type)
Preparation:wholemount
Procedures
General Information
Authors:Tang SJ; Suen TC; McInnes RR; Buchwald M, 1998 [PMID:9738002] . Indexed and spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1074/jbc.273.39.25356] [ PMID:9738002] Tang SJ, Suen TC, McInnes RR, Buchwald M 1998 Association of the TLX-2 homeodomain and 14-3-3eta signaling proteins. J Biol Chem (273):25356-63
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE