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EMAGE:5289

Lhx6 LIM homeobox protein 6 ( MGI:1306803)
TS21 (13.5 dpc)
in situ hybridisation

Data Images
EMAGE:5289 EMAGE:5289 EMAGE:5289 EMAGE:5289 EMAGE:5289
Raw signal output, as movie. All sections along the X-axis, as movie. All sections along the Y-axis, as movie. All sections along the Z-axis, as movie. Photograph prior to 3D imaging. Disssected Head
EMAGE:5289
Sample morphology following 3D imaging, as movie

View 3D opt image: EMAGE genex expression entry
Download 3D images in woolz format
Expression pattern clarity: three stars
Notes:
This is a traditionally produced wholemount in situ hybridisation stained using alkaline phosphatase + BM Purple. It has been imaged using Optical Projection Tomography. The visible channel depicting the signal has been false-coloured green and the fluorescence channel depicting autofluorescence has been false-coloured blue.
Expression Pattern Description
Text Annotation:
StructureLevelPatternNotes
palatal shelf
moderate moderate
graded
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:Lhx6_cloneB
Entity Detected:Lhx6, LIM homeobox protein 6 ( MGI:1306803)
Sequence:sense strand is shown

>Lhx6_cloneB
GGATCCACCACCTCTGTGTACACCCTCACCCGCTGCTGTCCTGCCCTCTGCCAGTGGCCCCATCAACCCA
GGCCTGGTCTTTCCCTCTCCTGCTGAGAACCAGAACCCACCAGGAGCAGCAGAGTCCCTCTCTGGGAAGG
CAGGCTCCCTGAAATGTGGCATCAGCATGAAAACAAGCAGCCTGTCTTTCCTCATTAACTGGGAGTCCCA
CTTACCTACTGTCAGCTGATCACAGCAGCAATGGCTAATTCAGTCCGAGTCAACAACAGCCTCCTACTTC
ACAGGTTCTCTTCTCCCCCACATTGGCCTTTATTTACTACTTCCTTGGAACCATCTCCAAATTCCAAATA
GCCAACAACCCCCAACATTATATACTCTGCTGCTTTTGTCTGGAAAACTCTACAGTGTTTGTGGGATATC
CCCAAAGGAAAGCTGTGTTCTAATTTTATCATTTCCATCTGTCTGGTTATGTCAAGTTAACTCAGAAAAA
GAAGAGACACTGACCAACCCTGAGAGGCCCAACAGGGCAGAGATGGAGGCCTGCCCAGATGAAGAGGCAG
AGGATGGGCAGGGATGGGTGCCAAAGACCCCCCCCCCAATTGGCTTGGAGGAGAAGGACTGGGTACGCTG
GACTTGGAGAGGTGACGAGAGTTTCATGGAGAGAAAAGAGGAAACTTGGGGGGGGCGGAGTTTCCCCCAC
CCAATTACCTAGCAGGAGTCGTGGAAAGGATGGCGTCTGATCTGCACGATGACACCACTGAATGTTTAGG
TGGGCTAGCATTAGCGACCCTTGTTGGCCACTTTTCAGTCCTGAGATGAGGGCCTGAGCCAGTCTTCTCT
AGGGGTGGGATGAGACTACTCACGAGGACCAAGGAACAGAGCCCAGAGAAACAGCAGCCAGAATATTAGT
TCTGATTCATCCCCGAGGGAAGAGAGGTACCCATGGCTTCAGTGAGCACATGCCAAGCCCAGGATGCCAG
CTGGCACCTCTGCAATCTCCCC
nt 1360 - nt 2361 of NM_001083125.1
Notes:This Lhx6 probe fragment (1.5kb) was kindly donated by Dr.Abigail Tucker. The Lhx6 antisense probe was generated from template cut with NotI and transcribed from the T7 promoter site. Probe available on request.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:CD-1
Age:13.5 dpc
Theiler Stage:TS21
Mutations:none (wild-type)
Preparation:opt
Procedures
Fixation:4% paraformaldehyde
Embedding:agarose
Staining procedure:alkaline phosphatase + BMpurple
General Information
Authors:Laura Ward, Mike Dixon
Principal investigator:Mike Dixon, University of Manchester, Faculty of Life Sciences Michael Smith Building Oxford Road, Manchester, England M13 9PT
Submitted by:Laura Ward, University of Manchester, Faculty of Life Sciences Michael Smith Building Oxford Road, Manchester, England M13 9PT
Experiment type:screen
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceFACEBASE