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EMAGE:5293

Dusp6 dual specificity phosphatase 6 ( MGI:1914853)
TS20 (12.5 dpc)
in situ hybridisation

Data Images
EMAGE:5293 EMAGE:5293 EMAGE:5293 EMAGE:5293 EMAGE:5293
Raw signal output, as movie. All sections along the X-axis, as movie. All sections along the Y-axis, as movie. All sections along the Z-axis, as movie. Photograph prior to 3D imaging.
EMAGE:5293 EMAGE:5293 EMAGE:5293
Photograph prior to 3D imaging. Photograph prior to 3D imaging. Pyst1 expression in the vibrissae and surrounding primitive anterior naris. Possible expression in the anterior palatal shelves. Sample morphology following 3D imaging, as movie

View 3D opt image: EMAGE genex expression entry
Download 3D images in woolz format
Expression pattern clarity: three stars
Notes:
This is a traditionally produced wholemount in situ hybridisation stained using alkaline phosphatase + BM Purple. It has been imaged using Optical Projection Tomography. The visible channel depicting the signal has been false-coloured yellow and the fluorescence channel depicting autofluorescence has been false-coloured blue.
Expression Pattern Description
Text Annotation:
StructureLevelPatternNotes
medial-nasal process epithelium
moderate moderate
regional
latero-nasal process epithelium
moderate moderate
regional
palatal shelf
possible possible
regional
vibrissa
strong strong
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:Dusp6_probeA
Entity Detected:Dusp6, dual specificity phosphatase 6 ( MGI:1914853)
Notes:The Dusp6 (Pyst1/Mkp3) probe template fragment used is 686bp long and cloned in pBluescript. The antisense probe was produced from template cut with XbaI and transcribed with T7 polymerase. Probe available on request.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:CD-1
Age:12.5 dpc
Theiler Stage:TS20
Mutations:none (wild-type)
Preparation:opt
Procedures
Fixation:4% paraformaldehyde
Embedding:agarose
Staining procedure:alkaline phosphatase + BMpurple
General Information
Authors:Laura Ward, Mike Dixon
Principal investigator:Mike Dixon, University of Manchester, Faculty of Life Sciences Michael Smith Building Oxford Road, Manchester, England M13 9PT
Submitted by:Laura Ward, University of Manchester, Faculty of Life Sciences Michael Smith Building Oxford Road, Manchester, England M13 9PT
Experiment type:screen
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceFACEBASE