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EMAGE:5300

Erbb4 v-erb-a erythroblastic leukemia viral oncogene homolog 4 (avian) ( MGI:104771)
TS15 (9.5 dpc)
in situ hybridisation

Data Images
EMAGE:5300
Fig2A [doi:10.1038/378390a0] Nature 1995 378(6555): 390-394, Gassmann M; Casagranda F; Orioli D; Simon H; Lai C; Klein R; Lemke G, Aberrant neural and cardiac development in mice lacking the ErbB4 neuregulin receptor. 1995 [PMID:7477376] © Nature Publishing Group. Reproduced with permission from Nature.

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Image annotations: Hb - hindbrain, H - heart.
Expression Pattern Description
Spatial Annotation:
EMAGE:5300Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
5300_wholemount__moderate.wlz
5300_wholemount__notDetected.wlz
5300_wholemount__strong.wlz
5300_wholemount__weak.wlz
5300_wholemount__possible.wlz
(what is wlz format?)
Download all expression domains: EMAGE:5300_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
heart
detected detected
regional
central nervous system
detected detected
regionalErbB4 mRNA is evident in the rostral CNS.
future hindbrain
detected detected
regionalTwo stripes of expression are evident in segments of the hindbrain.
rhombomere 03
detected detected
regionalErbB4 mRNA is confined dorsally
rhombomere 05
detected detected
regionalErbB4 mRNA is confined dorsally
primitive ventricle endocardial lining
not detected not detected
homogeneousErbB4 mRNA is not detected in the endocardium that lines the ventricle.
primitive ventricle cardiac muscle
detected detected
ErbB4 mRNA is detectable in the myocardium of the ventricle.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:Erbb4 probeA
Entity Detected:Erbb4, v-erb-a erythroblastic leukemia viral oncogene homolog 4 (avian) ( MGI:104771)
Notes:The Erbb4 probe template used in this study by Gassmann et al., 1995 [PMID:7477376] for whole-mount ISH is not described, however "a 5' mouse ErbB4 cDNA fragment subcloned into pCR-Script" was used to generate radiolabelled probe for section ISH in the same study.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Age:9.5 dpc
Theiler Stage:TS15
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Staining procedure:alkaline phosphatase + unspecified
General Information
Authors:Gassmann M; Casagranda F; Orioli D; Simon H; Lai C; Klein R; Lemke G, 1995 [PMID:7477376] , Indexed and Spatially Mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1038/378390a0] [ PMID:7477376] Gassmann M, Casagranda F, Orioli D, Simon H, Lai C, Klein R, Lemke G 1995 Aberrant neural and cardiac development in mice lacking the ErbB4 neuregulin receptor. Nature (378):390-4
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE