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EMAGE:5302

Pax9 paired box gene 9 ( MGI:97493)
TS17 (10.5 dpc)
in situ hybridisation

Data Images
EMAGE:5302 EMAGE:5302 EMAGE:5302 EMAGE:5302 EMAGE:5302
Raw signal output, as movie. All sections along the X-axis, as movie. All sections along the Y-axis, as movie. All sections along the Z-axis, as movie. Photograph prior to 3D imaging.
EMAGE:5302 EMAGE:5302 EMAGE:5302
Photograph prior to 3D imaging. Photograph prior to 3D imaging. Sample morphology following 3D imaging, as movie

View 3D opt image: EMAGE genex expression entry
Download 3D images in woolz format
Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
This is a traditionally produced wholemount in situ hybridisation stained using alkaline phosphatase + BM Purple. It has been imaged using Optical Projection Tomography. The visible channel depicting the signal has been false-coloured yellow and the fluorescence channel depicting autofluorescence has been false-coloured blue.
Expression Pattern Description
Spatial Annotation:
EMAGE:5302Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
5302_wholemount_moderate_3D_1.wlz
5302_wholemount_weak_3D_1.wlz
5302_wholemount_possible_3D_1.wlz
5302_wholemount_notDetected_3D_1.wlz
5302_wholemount_strong_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:5302_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
medial-nasal process
strong strong
homogeneous
trunk somite
moderate moderate
regional
3rd branchial groove
strong strong
regional
4th branchial groove
strong strong
regional
forelimb bud
weak weak
regional
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:Pax9_cloneB
Entity Detected:Pax9, paired box gene 9 ( MGI:97493)
Notes:This probe template (insert size 291bp) was cloned into pBluescript II KS (for reference see Neubuser et al. (1995) [PMID:7649395] ). To generate Pax9 antisense probe, the plasmid was cut with BamH I and transcribed from the T3 promoter. Template clone available on request.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:CD-1
Age:10.5 dpc
Theiler Stage:TS17
Mutations:none (wild-type)
Preparation:opt
Procedures
Fixation:4% paraformaldehyde
Embedding:agarose
Staining procedure:alkaline phosphatase + BMpurple
General Information
Authors:Bozena Palinkasova, Mike Dixon
Principal investigator:Mike Dixon, University of Manchester, Faculty of Life Sciences Michael Smith Building Oxford Road, Manchester, England M13 9PT
Submitted by:Bozena Palinkasova, University of Manchester, Faculty of Life Sciences Michael Smith Building Oxford Road, Manchester, England M13 9PT
Experiment type:screen
References:[ doi:10.1006/dbio.1995.1248] [ PMID:7649395] Neubuser A, Koseki H, Balling R 1995 Characterization and developmental expression of Pax9, a paired-box-containing gene related to Pax1. Dev Biol (1995):701-16
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceFACEBASE