Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:5306

Ext1 exostoses (multiple) 1 ( MGI:894663)
TS17 (10.5 dpc)
in situ hybridisation

Data Images
EMAGE:5306 EMAGE:5306
Fig2D. [doi:10.1006/bbrc.1998.9050] Reprinted from Biochem Biophys Res Comm, vol 248(3), Lin X; Gan L; Klein WH; Wells D, Expression and functional analysis of mouse EXT1, a homolog of the human multiple exostoses type 1 gene. pp 738-43, Copyright (1998) with permission from Elsevier. [PMID:9703997] Fig2A. [doi:10.1006/bbrc.1998.9050] Reprinted from Biochem Biophys Res Comm, vol 248(3), Lin X; Gan L; Klein WH; Wells D, Expression and functional analysis of mouse EXT1, a homolog of the human multiple exostoses type 1 gene. pp 738-43, Copyright (1998) with permission from Elsevier. [PMID:9703997]

Expression pattern clarity: two stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
2D - hybridised with antisense probe, 2A - hybridised with sense probe.
Expression Pattern Description
Spatial Annotation:
EMAGE:5306Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
5306_wholemount__moderate.wlz
5306_wholemount__notDetected.wlz
5306_wholemount__strong.wlz
(what is wlz format?)
Download all expression domains: EMAGE:5306_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
limb
detected detected
regionalEXT1 expression appeared most intensely at the distal and peripheral regions of the limb buds.
tail
detected detected
EXT1 expression appeared in the tail bud.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:Ext1 probeA
Entity Detected:Ext1, exostoses (multiple) 1 ( MGI:894663)
Notes:The Ext1 probe used in this study by Lin et al., 1998 [PMID:9703997] is described as follows "Plasmid LP5 was constructed by inserting a 0.92 kb 5' fragment (nt 342-1260) of the mEXT1 cDNA into pBluescript II (Stratagene). DIG-11-UTP-labeled antisense RNA probe was synthesized by T7 polymerase using XbaI digested plasmid LP5 as template." Editors Note: X96639.1 is the mEXT1 cDNA sequence submitted to GenBank by Lin,X. however it is unclear if the authors are referring to this sequence as other co-ordinates given in the paper for primers etc, do not exactly correlate.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:10.5 dpc
Theiler Stage:TS17
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
General Information
Authors:Lin X; Gan L; Klein WH; Wells D, 1998 [PMID:9703997] , Indexed and Spatially Mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1006/bbrc.1998.9050] [ PMID:9703997] Lin X, Gan L, Klein WH, Wells D 1998 Expression and functional analysis of mouse EXT1, a homolog of the human multiple exostoses type 1 gene. Biochem Biophys Res Commun (248):738-43
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE