Type: | in situ hybridisation probe |
Identifier: | Ext1 probeA |
Entity Detected: | Ext1, exostoses (multiple) 1 ( MGI:894663) |
Notes: | The Ext1 probe used in this study by Lin et al., 1998 [PMID:9703997] is described as follows "Plasmid LP5 was constructed by inserting a 0.92 kb 5' fragment (nt 342-1260) of the mEXT1 cDNA into pBluescript II (Stratagene). DIG-11-UTP-labeled antisense RNA probe was synthesized by T7 polymerase using XbaI digested plasmid LP5 as template."
Editors Note: X96639.1 is the mEXT1 cDNA sequence submitted to GenBank by Lin,X. however it is unclear if the authors are referring to this sequence as other co-ordinates given in the paper for primers etc, do not exactly correlate. |
Chemistry: | RNA |
Strand: | antisense |
Label: | digoxigenin |