Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:5308

Pax9 paired box gene 9 ( MGI:97493)
TS19 (11.5 dpc)
in situ hybridisation

Data Images
EMAGE:5308 EMAGE:5308 EMAGE:5308 EMAGE:5308 EMAGE:5308
Raw signal output, as movie. All sections along the X-axis, as movie. All sections along the Y-axis, as movie. All sections along the Z-axis, as movie. Photograph prior to 3D imaging.
EMAGE:5308 EMAGE:5308 EMAGE:5308
Photograph prior to 3D imaging. Photograph prior to 3D imaging. Sample morphology following 3D imaging, as movie

View 3D opt image: EMAGE genex expression entry
Download 3D images in woolz format
Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
This is a traditionally produced wholemount in situ hybridisation stained using alkaline phosphatase + BM Purple. It has been imaged using Optical Projection Tomography. The visible channel depicting the signal has been false-coloured green and the fluorescence channel depicting autofluorescence has been false-coloured blue.
Expression Pattern Description
Spatial Annotation:
EMAGE:5308Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
5308_wholemount__moderate.wlz
5308_wholemount__weak.wlz
5308_wholemount__notDetected.wlz
5308_wholemount__strong.wlz
(what is wlz format?)
Download all expression domains: EMAGE:5308_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
trunk somite
moderate moderate
regional
tail
strong strong
regional
future arm
strong strong
regional
handplate
strong strong
regional
footplate
moderate moderate
regional
1st branchial groove
strong strong
homogeneous
mandibular process
strong strong
regional
latero-nasal process
moderate moderate
regional
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:Pax9_cloneB
Entity Detected:Pax9, paired box gene 9 ( MGI:97493)
Notes:This probe template (insert size 291bp) was cloned into pBluescript II KS (for reference see Neubuser et al. (1995) [PMID:7649395] ). To generate Pax9 antisense probe, the plasmid was cut with BamH I and transcribed from the T3 promoter. Template clone available on request.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:CD-1
Age:11.5 dpc
Theiler Stage:TS19
Mutations:none (wild-type)
Preparation:opt
Procedures
Fixation:4% paraformaldehyde
Embedding:agarose
Staining procedure:alkaline phosphatase + BMpurple
General Information
Authors:Bozena Palinkasova, Mike Dixon
Principal investigator:Mike Dixon, University of Manchester, Faculty of Life Sciences Michael Smith Building Oxford Road, Manchester, England M13 9PT
Submitted by:Bozena Palinkasova, University of Manchester, Faculty of Life Sciences Michael Smith Building Oxford Road, Manchester, England M13 9PT
Experiment type:screen
References:[ doi:10.1006/dbio.1995.1248] [ PMID:7649395] Neubuser A, Koseki H, Balling R 1995 Characterization and developmental expression of Pax9, a paired-box-containing gene related to Pax1. Dev Biol (1995):701-16
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceFACEBASE