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EMAGE:5310

Eya2 eyes absent 2 homolog (Drosophila) ( MGI:109341)
TS15 (9.5 dpc)
in situ hybridisation

Data Images
EMAGE:5310
Fig 3C. Copyright: This image is from Xu PX; Woo I; Her H; Beier DR; Maas RL, Development 1997;124(1):219-31 and is displayed with the permission of The Company of Biologist Limited who owns the Copyright. [PMID:9006082]

Expression pattern clarity: two stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Image annotations: e - eye; b - brain; gV - trigeminal ganglion; gVII-VIII - facioacoustic ganglion complex; drg; dorsal root ganglia; arrows indicate expression in the epibranchial placodes.
Expression Pattern Description
Spatial Annotation:
EMAGE:5310Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
5310_wholemount__moderate.wlz
5310_wholemount__possible.wlz
5310_wholemount__notDetected.wlz
5310_wholemount__strong.wlz
5310_wholemount__weak.wlz
(what is wlz format?)
Download all expression domains: EMAGE:5310_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
future brain
detected detected
dorsal root ganglion
detected detected
trunk somite
detected detected
embryo
detected detected
regionalEya2 expression is detected in all ectodermal cranial placodes and placode derivatives. Eya2 is expressed in the epibranchial placodes and their cranial ganglia derivatives.
facio-acoustic vii-viii preganglion complex
detected detected
glossopharyngeal ix preganglion
detected detected
glossopharyngeal-vagus ix-x preganglion complex
detected detected
vagus x preganglion
detected detected
trigeminal v preganglion
detected detected
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:Eya2 probeB
Entity Detected:Eya2, eyes absent 2 homolog (Drosophila) ( MGI:109341)
Notes:The Eya2 probe used in this study by Xu et al., 1997 [PMID:9006082] is described as "Eya2 cDNA insert in pBluescript II KS+". Editors note: an Eya2 cDNA is shown in Fig1B therein and designated as U61111 by the authors, however the sequence shown in Fig1B is 2173 in length and U61111.1 is 2113bp in length and the 5' end sequences differ. It is unclear which specific Eya2 cDNA sequence the authors refer to.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Age:9.5 dpc
Theiler Stage:TS15
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:0.1% Triton X-100/3.7% formaldehyde in PBS
Staining procedure:alkaline phosphatase + unspecified
General Information
Authors:Xu PX; Woo I; Her H; Beier DR; Maas RL, 1997 [PMID:9006082] , Indexed and Spatially Mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ PMID:9006082] Xu PX, Woo I, Her H, Beier DR, Maas RL 1997 Mouse Eya homologues of the Drosophila eyes absent gene require Pax6 for expression in lens and nasal placode. Development (124):219-31
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE