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EMAGE:5316

Eya4 eyes absent 4 homolog (Drosophila) ( MGI:1337104)
TS17 (10.5 dpc)
in situ hybridisation

Data Images
EMAGE:5316
Fig7C. from Hum Mol Genet 1999 8(1): 11-23, Borsani G; DeGrandi A; Ballabio A; Bulfone A; Bernard L; Banfi S; Gattuso C; Mariani M; Dixon M; Donnai D; Metcalfe K; Winter R; Robertson M; Axton R; Brown A; van Heyningen V; Hanson I, EYA4, a novel vertebrate gene related to Drosophila eyes absent. Copyright 1999 [PMID:9887327]

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
The authors state that in Fig7C "the signal in the hindbrain (hb) is due to trapping", hence the hindbrain has not been annotated in this EMAGE entry..
Expression Pattern Description
Spatial Annotation:
EMAGE:5316Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
5316_wholemount__moderate.wlz
5316_wholemount__notDetected.wlz
5316_wholemount__strong.wlz
5316_wholemount__weak.wlz
5316_wholemount__possible.wlz
(what is wlz format?)
Download all expression domains: EMAGE:5316_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
1st branchial arch mandibular component
detected detected
forelimb bud
detected detected
hindlimb bud
detected detected
latero-nasal process
detected detected
eye
not detected not detected
homogeneous
embryo
detected detected
regionalExpression is detected in a region "in the vicinity of the somites".
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:1337368
Entity Detected:Eya4, eyes absent 4 homolog (Drosophila) ( MGI:1337104)
Sequence:sense strand is shown

>MGI:1337368
AGGAAGCTGGCTTTTCGTTACAGAAGAGTAAAGGAATTATATAACACCTACAAGAACAATGTTGGAGGAC
TCCTTGGCCCTGCTAAGAGGGATGCATGGCTGCAGTTAAGGGCAGAGATCGAAGGCCTAACAGATTCCTG
GCTAACGAATGCACTTAAGTCTTTATCAATTATTAGTACTAGGAGTAACTGTGTAAATGTCTTGGTAACG
ACAACCCAGCTGATTCCTGCTCTTGCAAAAGTCCTGCTCTACAGCCTAGGAGGTGCTTTCCCCATTGAGA
ACATTTACAGCGCAACGAAAATAGGCAAGGAAAGCTGTTTTGAGCGTATAGTGTCCAGATTTGGCACTAA
CATAACT
nt 1 - nt 357 of AJ007997.1
Notes:The Eya4 probe used in this study by Borsani et al., 1999 [PMID:9887327] is described as being "prepared from the mouse Eya4 RT-PCR clone MRT11 (Fig1, m-eya4)." Editors Note: In the Fig1 legend, the authors state that the nucleotide sequence of the RT-PCR product for m-eya4 has the accession number AJ007997.
Chemistry:RNA
Strand:sense
Label:digoxigenin
Specimen
Organism:mouse
Age:10.5 dpc
Theiler Stage:TS17
Mutations:none (wild-type)
Preparation:wholemount
Procedures
General Information
Authors:Borsani G; DeGrandi A; Ballabio A; Bulfone A; Bernard L; Banfi S; Gattuso C; Mariani M; Dixon M; Donnai D; Metcalfe K; Winter R; Robertson M; Axton R; Brown A; van Heyningen V; Hanson I, 1999 [PMID:9887327] , , Indexed by GXD, Spatially Mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1093/hmg/8.1.11] [ PMID:9887327] Borsani G, DeGrandi A, Ballabio A, Bulfone A, Bernard L, Banfi S, Gattuso C, Mariani M, Dixon M, Donnai D, Metcalfe K, Winter R, Robertson M, Axton R, Brown A, van Heyningen V, Hanson I 1999 EYA4, a novel vertebrate gene related to Drosophila eyes absent. Hum Mol Genet (8):11-23
Links:MGI:3760135 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI