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EMAGE:5323

Fabp7 fatty acid binding protein 7, brain ( MGI:101916)
TS17 (10.5 dpc)
in situ hybridisation

Data Images
EMAGE:5323
Fig1B(wt). Copyright: Reprinted with permission from the Society for Neuroscience from [doi:10.1523/JNEUROSCI.2512-05.2005] J Neurosci 25(42): 9752-61. Arai Y; Funatsu N; Numayama-Tsuruta K; Nomura T; Nakamura S; Osumi N. Role of Fabp7, a downstream gene of Pax6, in the maintenance of neuroepithelial cells during early embryonic development of the rat cortex. Copyright 2005. [PMID:16237179]

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
The image shows a whole-mount in situ hybridisation of Fabp7 in the dissected brain tube of wild-type mouse at E10.5.
Expression Pattern Description
Spatial Annotation:
EMAGE:5323Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
5323_wholemount__moderate.wlz
5323_wholemount__weak.wlz
5323_wholemount__notDetected.wlz
5323_wholemount__strong.wlz
(what is wlz format?)
Download all expression domains: EMAGE:5323_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
telencephalon
strong strong
regionalFabp7 is intensely expressed in the ventral telencephalon of E10.5 mouse embryos, and not specifically expressed in the dorsal telencephalon (where rat Fabp7 is expressed - see Fig 1A).
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:Fabp7 probeA
Entity Detected:Fabp7, fatty acid binding protein 7, brain ( MGI:101916)
Sequence:sense strand is shown

>Fabp7 probeA
GATGCTTTCTGTGCCACCTGGAAGCTGACAGACAGCCAGAATTTTGATGAGTACATGAAGGCTCTGGGCG
TGGGCTTTGCCACTAGGCAAGTGGGAAATGTGACCAAACCAACGGTGATTATCAGTCAGGAAGGCGGCAA
AGTGGTGATCCGGACACAATGCACATTCAAGAACACAGAGATCAGTTTCCAGCTGGGAGAAGAGTTTGAG
GAAACAAGCATAGATGACAGGAACTGTAAGTCTGTGATTCGGTTGGATGGAGACAAGCTCATTCATGTGC
AGAAGTGGGATGGCAAAGAAACAAATTGTGTCAGAGAAATTAAGGATGGCAAAATGGTTGTGACTCTCAC
CTTTGGGGATGTCGTTGCTGTTCGCTGTTATGAAAAGGCATAGAAGAAATGCCGGGTCTTTTGAAAACTT
GGTTTCCTGAAGTTCCAGAGTTATCCAGCTACTGAAGCACTGGTCACTAATTATAAGTTTGTCTTTGGTG
TGGAGGCAG
nt 89 - nt 587 of NM_030832.1
Notes:The Fabp7 probe template used in this study by Arai et al., 2005 [PMID:16237179] was a "cDNA for rat Fabp7", which "was amplified by PCR with the same dsDNA templates and primers used for quantitative PCR (5'-GATGCTTTCTGTGCCACCTG-3' and 5'-CTGCCTCCACACCAAAGACA-3'), and the obtained cDNA fragment was cloned into pBluescriptIISK(-) (Stratagene, La Jolla, CA)".
Chemistry:RNA
Strand:antisense
Specimen
Organism:mouse
Age:10.5 dpc
Theiler Stage:TS17
Mutations:none (wild-type)
Preparation:wholemount
Procedures
General Information
Authors:Arai Y; Funatsu N; Numayama-Tsuruta K; Nomura T; Nakamura S; Osumi N, 2005 [PMID:16237179] , Indexed and Spatially Mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1523/JNEUROSCI.2512-05.2005] [ PMID:16237179] Arai Y, Funatsu N, Numayama-Tsuruta K, Nomura T, Nakamura S, Osumi N 2005 Role of Fabp7, a downstream gene of Pax6, in the maintenance of neuroepithelial cells during early embryonic development of the rat cortex. J Neurosci (25):9752-61
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE