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EMAGE:5325

Fasn fatty acid synthase ( MGI:95485)
TS15 (9.5 dpc)
in situ hybridisation

Data Images
EMAGE:5325 EMAGE:5325
Fig5(e9.5-antisense). Copyright: This image is from Chirala SS; Chang H; Matzuk M; Abu-Elheiga L; Mao J; Mahon K; Finegold M; Wakil SJ, Proc Natl Acad Sci U S A 2003 May 27;100(11):6358-63. [PMID:12738878] Copyright 2003 National Academy of Sciences, U.S.A. Fig5(e9.5-sense). Copyright: This image is from Chirala SS; Chang H; Matzuk M; Abu-Elheiga L; Mao J; Mahon K; Finegold M; Wakil SJ, Proc Natl Acad Sci U S A 2003 May 27;100(11):6358-63. [PMID:12738878] Copyright 2003 National Academy of Sciences, U.S.A.

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Image annotations: np, nasal pit; b, branchial arches; lb, limb bud. One of these embryos was hybridised with antisense probe and one with sense probe (as indicated in the Figure legends).
Expression Pattern Description
Spatial Annotation:
EMAGE:5325Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
5325_wholemount__moderate.wlz
5325_wholemount__weak.wlz
5325_wholemount__notDetected.wlz
5325_wholemount__strong.wlz
(what is wlz format?)
Download all expression domains: EMAGE:5325_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
surface ectoderm
detected detected
Labeling was apparent throughout the epidermis.
forelimb bud ectoderm
strong strong
regionalThe strongest labeling was seen in the distal epidermis of the limb bud, including the apical ectodermal ridge, an important signaling center for limb outgrowth, and its underlying mesenchyme.
forelimb bud mesenchyme
strong strong
The strongest labeling was seen in the distal epidermis of the limb bud, including the apical ectodermal ridge, an important signaling center for limb outgrowth, and its underlying mesenchyme.
hindlimb ridge ectoderm
strong strong
regionalThe strongest labeling was seen in the distal epidermis of the limb bud, including the apical ectodermal ridge, an important signaling center for limb outgrowth, and its underlying mesenchyme.
hindlimb ridge mesenchyme
strong strong
The strongest labeling was seen in the distal epidermis of the limb bud, including the apical ectodermal ridge, an important signaling center for limb outgrowth, and its underlying mesenchyme.
branchial arch
strong strong
regionalThe distal portions of the branchial arches were strongly labeled.
fronto-nasal process
strong strong
regionalThe distal portions of the frontonasal mass were strongly labeled.
neural tube
weak weak
The closed neural tube was not strongly labeled.
embryo
detected detected
the FAS gene was broadly expressed in the mouse embryo.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:Fasn probeA
Entity Detected:Fasn, fatty acid synthase ( MGI:95485)
Notes:The Fasn probe used in this study by Chirala et al., 2003 [PMID:12738878] is of unreported sequence.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Age:9.5 dpc
Theiler Stage:TS15
Mutations:none (wild-type)
Preparation:wholemount
Procedures
General Information
Authors:Chirala SS; Chang H; Matzuk M; Abu-Elheiga L; Mao J; Mahon K; Finegold M; Wakil SJ, 2003 [PMID:12738878] , Indexed and Spatially Mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1073/pnas.0931394100] [ PMID:12738878] Chirala SS, Chang H, Matzuk M, Abu-Elheiga L, Mao J, Mahon K, Finegold M, Wakil SJ 2003 Fatty acid synthesis is essential in embryonic development: fatty acid synthase null mutants and most of the heterozygotes die in utero. Proc Natl Acad Sci U S A (100):6358-63
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE