EMAGE mouse gene expression spatial database

EMAGE:5327

Fbl fibrillarin ( MGI:95486)
TS15 (9.5 dpc)
in situ reporter

Data Images


Fig5A(9.5) of [doi:10.1128/MCB.23.23.8519-8527.2003] Mol Cell Biol 2003 23(23):8519-8527 [PMID:14612397] Newton K; Petfalski E; Tollervey D; Caceres JF, "Fibrillarin is essential for early development and required for accumulation of an intron-encoded small nucleolar RNA in the mouse." � ASM. Reproduced with permission from Molecular and Cellular Biology. Reuse of any Licensed Material requires permission from ASM. Copyright 2003.

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Expression Pattern Description

Spatial Annotation:

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Text Annotation:

Structure

Level

Pattern

Notes

embryo

detected

Fibrillarin expression, as measured by beta-galactosidase activity, "was ubiquitous until 11.5 dpc".

Annotation Validation:

EMAGE Editor

Detection Reagent

Type:	in situ reporter
Entity Detected:	Fbl, fibrillarin ( MGI:95486)
Notes:	Fbl (Fibrillarin) expression was assayed in this study by Newton et al., 2003 [PMID:14612397] in embryo heterozygous for a gene trapped allele, where the endogenous locus was disrupted by insertion into intron 4 of the pGT1,-2, or-3 retroviral gene trap vector containing a splice acceptor site, beta-geo, and an SV40 poly-A signal. A fusion protein consisting of the N-terminal 132 amino acids of the Fbl protein fused to lacZ is expressed from the mutated locus.
Labelled with:	LacZ

Specimen

Procedures

Fixation:

0.5% glutaraldehyde

General Information

Authors:	Newton K; Petfalski E; Tollervey D; Caceres JF, 2003 [PMID:14612397] , Indexed and Spatially Mapped by EMAGE.
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	non-screen
References:	[ doi:10.1128/MCB.23.23.8519-8527.2003] [ PMID:14612397] Newton K, Petfalski E, Tollervey D, Caceres JF 2003 Fibrillarin is essential for early development and required for accumulation of an intron-encoded small nucleolar RNA in the mouse. Mol Cell Biol (2003):8519-27
Links:	MGI:3043754 allele description
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source