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EMAGE:5330

Fbxl16 F-box and leucine-rich repeat protein 16 ( MGI:2448488)
TS16 (10 dpc)
in situ hybridisation

Data Images
EMAGE:5330
Fig3(e10). from Exp Mol Med 2007 39(3): 255-66, Liu T; Ma Z; Que H; Li X; Ni Y; Jing S; Liu S, Identification and characterization of scirr1, a novel gene up-regulated after spinal cord injury. Copyright 2001 [PMID:17603280]

Expression pattern clarity: two stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Authors state that "KM" mouse embryos were used.
Expression Pattern Description
Spatial Annotation:
EMAGE:5330Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
5330_wholemount__moderate.wlz
5330_wholemount__possible.wlz
5330_wholemount__notDetected.wlz
5330_wholemount__strong.wlz
(what is wlz format?)
Download all expression domains: EMAGE:5330_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
telencephalon
detected detected
regionalExpression in ventral aspects of telencephalon.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:3763128
Entity Detected:Fbxl16, F-box and leucine-rich repeat protein 16 ( MGI:2448488)
Sequence:sense strand is shown

>MGI:3763128
TCGTGCTGGACAGGTGTGTACGCATCACGGACACTGGTCTCAGCTACTTGTCCACCATGTCGTCCCTCCG
CAGCCTCTACCTGCGATGGTGCTGCCAGGTGCAGGACTTCGGGTTGAAGCACCTCTTAGCCATGAGAAGT
TTGCGAC
nt 1130 - nt 1276 of NM_001009504.1
Notes:The Fbxl16 (scirr1) probe template used in this study by Liu et al., 2007 [PMID:17603280] is described as follows: "Blast search against the rat mRNA database of GenBank revealed that the 147 bp fragment from nucleotides 1,130 to 1,276 is the most specific sequence of the rat scirr1 coding region. This fragment covers the 3' part of the third exon and the 5' part of the fourth exon, which enables probe derived from this fragment to identify scirr1 mRNA specifically. Then this fragment was amplified using primers 5'-CTGGAATTCGTCGCAAACTTCTCATGGCTAA-3' and 5'-TACAAGCTTTCGTGCTGGACAGGTGTGTAC-3', and the PCR product was inserted into the pSPT19 vector (Roche, Grenzacherstrasse, Basel, Switzerland) between the EcoRI and HindIII sites." Editors Note: It is assumed the template sequence was amplified from a rat mRNA source, although it is not specifically stated by the authors.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Age:10 dpc
Theiler Stage:TS16
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Liu T; Ma Z; Que H; Li X; Ni Y; Jing S; Liu S, 2007 [PMID:17603280] , Indexed by GXD, Spatially Mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1038/emm.2007.29] [ PMID:17603280] Liu T, Ma Z, Que H, Li X, Ni Y, Jing S, Liu S 2007 Identification and characterization of scirr1, a novel gene up-regulated after spinal cord injury. Exp Mol Med (39):255-66
Links:MGI:3763135 same experiment
 MGI:3763126 primer sequences
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI