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EMAGE:5334

Satb2 special AT-rich sequence binding protein 2 ( MGI:2679336)
TS20 (12.5 dpc)
in situ hybridisation

Data Images
EMAGE:5334 EMAGE:5334 EMAGE:5334 EMAGE:5334 EMAGE:5334
Raw signal output, as movie. All sections along the X-axis, as movie. All sections along the Y-axis, as movie. All sections along the Z-axis, as movie. Photograph prior to 3D imaging.
EMAGE:5334 EMAGE:5334 EMAGE:5334
Photograph prior to 3D imaging. Photograph prior to 3D imaging. Sample morphology following 3D imaging, as movie

View 3D opt image: EMAGE genex expression entry
Download 3D images in woolz format
Expression pattern clarity: three stars
Notes:
This is a traditionally produced wholemount in situ hybridisation stained using alkaline phosphatase + BM Purple. It has been imaged using Optical Projection Tomography. The visible channel depicting the signal has been false-coloured green and the fluorescence channel depicting autofluorescence has been false-coloured blue.
Expression Pattern Description
Text Annotation:
StructureLevelPatternNotes
palatal shelf
strong strong
regional
maxillary process mesenchyme
strong strong
regional
Annotation Validation: Submitter + EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:Satb2_probeB
Entity Detected:Satb2, special AT-rich sequence binding protein 2 ( MGI:2679336)
Sequence:sense strand is shown

>Satb2_probeB
TGGTCTCCTCTGCCTCTAGCAGTCCCAGCTCCTCCCGAACCCCACAGGCCAAAACCTCGACACCGACAAC
AGACCTCCCTATTAAGGTGGACGGCGCCAACGTCAACATCACAGCTGCCATTTATGACGAGATCCAACAG
GAGATGAAAAGAGCCAAGGTGTCTCAAGCCCTGTTTGCCAAAGTGGCTGCAAACAAAAGTCAGGGCTGGC
TTTGCGAACTGCTTCGTTGGAAGGAGAACCCCAGCCCAGAAAACCGCACCCTTTGGGAGAATCTCTGCAC
CATCCGCCGTTTCCTGAATCTTCCCCAACATGAGCGGGATGTGATCTATGAGGAAGAATCTCGACATCAC
CACAGTGAACGCATGCAGCATGTGGTCCAGCTCCCACCTGAGCCCGTGCAGGTCCTTCATCGACAGCAGT
CCCAGCCAACTAAGGAGAGCTCCCCTCCCAGAGAAGAAGCACCCCCACCGCCTCCTCCAACAGAAGACAG
CTGTGCCAAA
nt 1622 - nt 2121 of NM_139146.2
Notes:An antisense probe template was cloned into Bluescript with a T7 promoter site. Antisense probe was generated using T7 polymerase from template linearised with BamH1. Primers used to generate the Satb2 clone: 5': tggtctcctctgcctctag 3' 3': tttggcacagctgtcttctg 5' Product length: 500 bp. Probe available upon request.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:CD-1
Age:12.5 dpc
Theiler Stage:TS20
Mutations:none (wild-type)
Preparation:opt
Procedures
Fixation:4% paraformaldehyde
Embedding:agarose
Staining procedure:alkaline phosphatase + BMpurple
General Information
Authors:Bozena Palinkasova, Mike Dixon
Principal investigator:Mike Dixon, University of Manchester, Faculty of Life Sciences Michael Smith Building Oxford Road, Manchester, England M13 9PT
Submitted by:Bozena Palinkasova, University of Manchester, Faculty of Life Sciences Michael Smith Building Oxford Road, Manchester, England M13 9PT
Experiment type:screen
References:[ doi:10.1093/hmg/ddg248] [ PMID:12915443] FitzPatrick DR, Carr IM, McLaren L, Leek JP, Wightman P, Williamson K, Gautier P, McGill N, Hayward C, Firth H, Markham AF, Fantes JA, Bonthron DT 2003 Identification of SATB2 as the cleft palate gene on 2q32-q33. Hum Mol Genet (12):2491-501
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceFACEBASE