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EMAGE:5343

Fgfr1 fibroblast growth factor receptor 1 ( MGI:95522)
TS16 (10 dpc)
in situ hybridisation

Data Images
EMAGE:5343
Fig1E. [doi:10.1242/dev.02001] Copyright: This image is from Verheyden JM; Lewandoski M; Deng C; Harfe BD; Sun X, Development 2005;132(19):4235-45 and is displayed with the permission of The Company of Biologist Limited who owns the Copyright. [PMID:16120640]

Expression pattern clarity: one star
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Image annotations: arrow indicates forelimb bud.
Expression Pattern Description
Spatial Annotation:
EMAGE:5343Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
5343_wholemount__moderate.wlz
5343_wholemount__weak.wlz
5343_wholemount__notDetected.wlz
5343_wholemount__strong.wlz
(what is wlz format?)
Download all expression domains: EMAGE:5343_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
forelimb bud mesenchyme
detected detected
Fgfr1 is widely expressed in the lateral plate mesoderm (LPM), including the limb bud mesenchyme (LBM) prior to as well as after limb initiation.
hindlimb bud mesenchyme
detected detected
Fgfr1 is widely expressed in the lateral plate mesoderm (LPM), including the limb bud mesenchyme (LBM) prior to as well as after limb initiation.
trunk mesenchyme
detected detected
regionalFgfr1 is widely expressed in the lateral plate mesoderm (LPM), including the limb bud mesenchyme (LBM) prior to as well as after limb initiation.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:Fgfr1 probeA
Entity Detected:Fgfr1, fibroblast growth factor receptor 1 ( MGI:95522)
Sequence:sense strand is shown

>Fgfr1 probeA
TCTGGAAGCCCTGGAAGAGAGACCAGCTGTGATGACCTCACCGCTCTACCTGGAGATCATTATCTACTGC
ACCGGGGCCTTCCTGATCTCCTGCATGTTGGGCTCTGTCATCATCTATAAGATGAAGAGCGGCACCAAGA
AGAGCGACTTCCATAGCCAGATGGCTGTGCACAAGCTGGCCAAGAGCATCCCTCTGCGCA
nt 1820 - nt 2019 of NM_010206.2
Notes:The Fgfr1 probe used in this study by Verheyden et al., 2005 [PMID:16120640] is described as follows: "The Fgfr1 in situ probe was prepared from a plasmid containing Fgfr1 exon 9 cDNA. This cDNA was generated by PCR using primer pair: 5'-TCTGGAAGCCCTGGAAGAGAGA-3' and 5'-TGCGCAGAGGGATGCTCTTG-3'."
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Age:10 dpc
Theiler Stage:TS16
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Staining procedure:alkaline phosphatase + BMpurple
General Information
Authors:Verheyden JM; Lewandoski M; Deng C; Harfe BD; Sun X, 2005 [PMID:16120640] , Indexed and Spatially Mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1242/dev.02001] [ PMID:16120640] Verheyden JM, Lewandoski M, Deng C, Harfe BD, Sun X 2005 Conditional inactivation of Fgfr1 in mouse defines its role in limb bud establishment, outgrowth and digit patterning. Development (132):4235-45
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE